ELIMINATION OF HOP LATENT VIROID (HLVd) IN HOP (Humulus lupulus L.) HYBRID 31/299

V diplomskem delu smo na Inštitutu za hmeljarstvo in pivovarstvo Slovenije v Žalcu raziskovali pridobivanje brezviroidnih rastlin iz meristemskega tkiva hmelja, križanca z oznako 31/299. Iz terminalnih in lateralnih brstov je bilo v sterilnih pogojih izoliranih 298 meristemov, ki smo jih položili na MS gojišče z 9µM BAP (6- benzilaminopurin) za iniciacijo brstov (MSB). Od izoliranih 298 meristemov jih je 58 propadlo, 40 jih je zakrnelo, 39 jih je kalusiralo. Od 161 ali 54 % ozelenelih in normalno regeneriranih meristemov jih 65 ali 22 % nismo testirali, 96 ali 33 % pa smo jih analizirali na prisotnost hmeljevega latentnega viroida (HLVd) z metodo RT-PCR in agarozno elektroforezo. Od opravljenih 96 preizkusov prisotnosti HLVd je bilo 9 brezviroidnih križancev hmelja z oznako 31/299. Viroide smo uspešno eliminirali pri 9 oziroma 3 % regenerantov. Eliminacija viroidov je bila uspešnejša pri glavnih poganjkih in manj uspešna pri stranskih poganjkih.The production of viroid-free hop plants of breeding line labeled 31/299 from meristem tissue was studied in the Graduation Thesis. Surveys have been done at the Slovenian Institute of Hop Research and Brewing in Žalec. From the terminal and lateral buds 298 meristems were asseptically isolated and placed on MSB medium for bud initiation with 9µM BAP(6- Benzylaminopurine). From isolated 298 meristems, 58 of them died, 40 of the meristems stunted and 39 callused. From 161 or 54 percentages of green and normal meristems, 65 or 22 percentages were not tested for the presence of HLVd viroid. 96 or 33 percentages of normal meristems were tested for the presence of viroid HLVd by RT-PCR method and agarose electrophoresis. We confirmed the effectiveness of used elimination HLVd method in 9 or 3 percentages of meristems. Elimination of HLVd was more successful in main stems rather than in lateral shoots