Development of in vitro plant and tissue cultures of common hop (Humulus lupulus) and characterization of antiradical and antimicrobial activity of its extracts

Darba mērķis bija iegūt parastā apiņa in vitro augu un audu (kallusu) kultūras un raksturot no tām, kā arī no lapu drogām, iegūtu ekstraktu antiradikālo un antimikrobiālo aktivitāti. In vitro augu un audu kultūru ieguvei tika ievākti trīs apiņa šķirņu paraugi. Analizējot kopējo fenolu saturu, antiradikālo un antimikrobiālo aktivitāti, tika savstarpēji salīdzināti drogu, in vitro augu un kallusu kultūru ekstrakti, kā arī izvērtētas efektivākās ekstrakcijas metodes. Kallusu kultūras tika iegūtas uz ar augšanas regulatoriem bagātinātas McCown Woody Plant barotnes. Lielāko fenolu savienojumu saturu un augstāko antiradikālo aktivitāti uzrādīja visu šķirņu drogu ekstrakti, vienlaikus antimikrobiālā aktivitāte tika novērota arī divu šķirņu in vitro augu un kallusu ekstraktiem. 30/30 etanola-glicerīna šķīdums tika konstatēts kā efektīvākais no izmantotajiem šķīdinātājiem.The aim of the thesis was to establish in vitro plant and tissue (callus) cultures of common hop and to characterize its as well as wild plant leaf extract antiradical and antimicrobial activities. To establish in vitro plant and tissue cultures, samples of three common hop varieties were collected. Different extraction methods of the plant material were compared as well as total phenolic content, antiradical and antimicrobial activity. Callus cultures were established on McCown Woody Plant medium supplimented with various growth regulators. Wild plant extracts possesed the highest total phenolic content and antiradical activity in all hop varieties. Antimicrobial activity was shown in all wild plant extracts as well as in callus and in vitro culture extracts of two hop varieties. 30/30 ethanol-glycerol solution was found to be the most efficient of the solvents used