We describe a Bayesian method for estimating the number of essential genes in a genome, on the basis of data on viable mutants for which a single transposon was inserted after a random TA site in a genome,potentially disrupting a gene. The prior distribution for the number of essential genes was taken to be uniform. A Gibbs sampler was used to estimate the posterior distribution. The method is illustrated with simulated data. Further simulations were used to study the performance of the procedure
Changes in nucleotide sequences, or mutations, accumulate from generation to generation in the genom...
Transposable elements (TEs) are interspersed DNA sequences that can move or copy to new positions wi...
This chapter introduces a set of transposon-based methods that were developed to sample trinucleotid...
Motivation Probabilistic Identification of bacterial essential genes using transposon-directed inse...
This is the final version of the article. Available from Springer Nature via the DOI in this record....
ABSTRACT For decades, identifying the regions of a bacterial chromosome that are necessary for viabi...
Large-scale systematic analysis of gene essentiality is an important step closer toward unraveling t...
Large-scale systematic analysis of gene essentiality is an important step closer toward unraveling t...
Transposon Sequencing (TnSeq) has become a popular biological tool for assessing the phenotypes of l...
The development of new technologies and approaches has vastly transformed genomics research, leading...
The thesis is composed of three independent projects: (i) analyzing transposon-sequencing data to in...
Abstract Background Tn-Seq is a high throughput techn...
Transposon insertion sequencing methods such as Tn-seq revolutionized microbiology by allowing the i...
Transposon-insertion sequencing (TIS) is a powerful approach for deciphering genetic requirements fo...
We have developed a versatile statistical analysis algorithm for the detection of genomic aberration...
Changes in nucleotide sequences, or mutations, accumulate from generation to generation in the genom...
Transposable elements (TEs) are interspersed DNA sequences that can move or copy to new positions wi...
This chapter introduces a set of transposon-based methods that were developed to sample trinucleotid...
Motivation Probabilistic Identification of bacterial essential genes using transposon-directed inse...
This is the final version of the article. Available from Springer Nature via the DOI in this record....
ABSTRACT For decades, identifying the regions of a bacterial chromosome that are necessary for viabi...
Large-scale systematic analysis of gene essentiality is an important step closer toward unraveling t...
Large-scale systematic analysis of gene essentiality is an important step closer toward unraveling t...
Transposon Sequencing (TnSeq) has become a popular biological tool for assessing the phenotypes of l...
The development of new technologies and approaches has vastly transformed genomics research, leading...
The thesis is composed of three independent projects: (i) analyzing transposon-sequencing data to in...
Abstract Background Tn-Seq is a high throughput techn...
Transposon insertion sequencing methods such as Tn-seq revolutionized microbiology by allowing the i...
Transposon-insertion sequencing (TIS) is a powerful approach for deciphering genetic requirements fo...
We have developed a versatile statistical analysis algorithm for the detection of genomic aberration...
Changes in nucleotide sequences, or mutations, accumulate from generation to generation in the genom...
Transposable elements (TEs) are interspersed DNA sequences that can move or copy to new positions wi...
This chapter introduces a set of transposon-based methods that were developed to sample trinucleotid...
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