This is the final version of the article. Available from Springer Nature via the DOI in this record.Massively parallel sequencing technology coupled with saturation mutagenesis has provided new and global insights into gene functions and roles. At a simplistic level, the frequency of mutations within genes can indicate the degree of essentiality. However, this approach neglects to take account of the positional significance of mutations - the function of a gene is less likely to be disrupted by a mutation close to the distal ends. Therefore, a systematic bioinformatics approach to improve the reliability of essential gene identification is desirable. We report here a parametric model which introduces a novel mutation feature together with a...
International audienceReverse transcription quantitative real-time polymerase chain reaction (Rt-qpC...
Large-scale systematic analysis of gene essentiality is an important step closer toward unraveling t...
Transposon-directed insertion-site sequencing (TraDIS) is a high-throughput method that couples tran...
This is the final version. Available from BMC via the DOI in this recordAvailability of data and mat...
ABSTRACT Rapid growth in deep tissue is essential to the high virulence of Yersinia pestis, causativ...
ABSTRACT Rapid growth in deep tissue is essential to the high virulence of Yersinia pestis, causativ...
Transposon-directed insertion site sequencing (TraDIS) is a high-throughput method coupling transpos...
BACKGROUND: Yersinia pseudotuberculosis is a zoonotic pathogen, causing mild gastrointestinal infect...
The development of new technologies and approaches has vastly transformed genomics research, leading...
Transposon mutagenesis is an efficient way to explore gene essentiality of a bacterial genome. Howev...
Abstract only availableTn5 transposon mutagenesis occurs by a mechanism in which a segment of DNA (t...
Transposon-directed insertion site sequencing (TraDIS) is a high-throughput method coupling transpos...
Transposon Sequencing (TnSeq) has become a popular biological tool for assessing the phenotypes of l...
Abstract only availableTn5 transposon mutagenesis occurs by a mechanism in which a segment of DNA (t...
Background. Yersinia pestis, the causative agent of plague, is responsible for some of the greatest ...
International audienceReverse transcription quantitative real-time polymerase chain reaction (Rt-qpC...
Large-scale systematic analysis of gene essentiality is an important step closer toward unraveling t...
Transposon-directed insertion-site sequencing (TraDIS) is a high-throughput method that couples tran...
This is the final version. Available from BMC via the DOI in this recordAvailability of data and mat...
ABSTRACT Rapid growth in deep tissue is essential to the high virulence of Yersinia pestis, causativ...
ABSTRACT Rapid growth in deep tissue is essential to the high virulence of Yersinia pestis, causativ...
Transposon-directed insertion site sequencing (TraDIS) is a high-throughput method coupling transpos...
BACKGROUND: Yersinia pseudotuberculosis is a zoonotic pathogen, causing mild gastrointestinal infect...
The development of new technologies and approaches has vastly transformed genomics research, leading...
Transposon mutagenesis is an efficient way to explore gene essentiality of a bacterial genome. Howev...
Abstract only availableTn5 transposon mutagenesis occurs by a mechanism in which a segment of DNA (t...
Transposon-directed insertion site sequencing (TraDIS) is a high-throughput method coupling transpos...
Transposon Sequencing (TnSeq) has become a popular biological tool for assessing the phenotypes of l...
Abstract only availableTn5 transposon mutagenesis occurs by a mechanism in which a segment of DNA (t...
Background. Yersinia pestis, the causative agent of plague, is responsible for some of the greatest ...
International audienceReverse transcription quantitative real-time polymerase chain reaction (Rt-qpC...
Large-scale systematic analysis of gene essentiality is an important step closer toward unraveling t...
Transposon-directed insertion-site sequencing (TraDIS) is a high-throughput method that couples tran...