PTX treatment increases surface expression of peptide-bound MHCII on DRG neurons from female mice.

(A) Representative flow cytometric dot plots of the frequency of DRG neurons expressing surface-MHCII from naïve and day 14 PTX-treated female mice. (B) Quantification of the frequency of DRG neurons from female (white bar, n = 9) and male (gray bar, n = 6) mice expressing surface-MHCII shown in (A). (C) Representative widefield fluorescence microscopy images of DRG neurons (PGP9.5+: red) expressing surface-MHCII (cyan) co-localized with OVA-FITC (yellow) from naïve and day 14 PTX-treated female and male mice. Diffuse (solid arrow) and punctate (dashed arrow) surface-MHCII staining in DRG neurons from naïve female and male mice. Quantification of (D) MHCII intensity, (E) OVA intensity, and (F) percent of neuron area with polarized MHCII from (C). (D-F) Statistical significance determined by 2-way ANOVA with Sidak’s multiple comparison test (*p <0.05, **p < 0.01, ****p < 0.0001, n = 3).