MHCII⁺ DRG neurons are primarily small diameter, and MHCII expression increases in female mice after PTX.

(A, E) Gaussian distribution of the diameter of MHCII+ DRG neurons in DRG tissue from naïve (pink), day 7 (orange) and day 14 (blue) PTX-treated (A) female and (E) male mice (n = 8, pooled neurons). Dashed black line: diameter mode of TRPV1-lineage td-tomato+ neurons (S6 Fig in S1 File). Quantification of (B, F) percent of small diameter (≤25 μm) DRG neurons positive for MHCII, (C, G) number of MHCII puncta/small diameter neuron, and (D, H) percent of small diameter neuron area with MHCII puncta. (B-D, F-H) Statistical significance determined by 1-way ANOVA with Dunnett’s multiple comparison test (*p <0.05, **p < 0.01, n = 8).