Additional file 1 of Wnt/β-catenin signalling activates IMPDH2-mediated purine metabolism to facilitate oxaliplatin resistance by inhibiting caspase-dependent apoptosis in colorectal cancer

Additional file 1: Figure S1. Network diagram of purine metabolic pathway. Small circle: metabolite (the red indicates that the metabolite is up-regulated, while the blue indicates that the metabolite is down-regulated in the comparison group); arrow: direction of response; small box: enzyme; large box: other metabolic pathways. Figure S2. IMPDH2 regulates CRC cell apoptosis in response to oxaliplatin. A and B Overexpression of IMPDH2 was confirmed at the mRNA and protein level in SW620 cells by qPCR and western blotting analysis. C Comparison of apoptosis induction between vector and IMPDH2-overexpression cells in SW620 after oxaliplatin treatment was carried out by flow cytometry analysis. Bar charts show the percentage of apoptotic and surviving cells, respectively. D The apoptotic rate was analysed after GMP and/or oxaliplatin treatment compared with that of untreated cells for 24 h by flow cytometry analysis in SW620 cells. E Western blotting analysis of apoptotic proteins expression was carried out between vector and IMPDH2-overexpression SW620 cells after oxaliplatin treatment. **p < 0.01, and ***p < 0.001. Figure S3. Network diagram of the Wnt signalling pathway. The small purple rectangle represents the uniquely proteins identified in HCT8/L-OHP cells