TNFα treated but not H₂O₂-induced senescent iAF cells show signs of degeneration at 24hrs.

(A) Gene expression analysis of iAF cells treated with TNFα (40 ng/mL). 18S rRNA Ct value (mean±SD): Control = = 9.37± 0.28, TNFα = 9.72±0.21. (B) Representative images of COL1, COL2, and ACAN immunocytochemistry of iAF cells treated with TNFα. (C) Quantification of immunocytochemistry in B, presented as percentage of total cells stained. (D) Gene expression of iAF cells treated with H2O2. 18S rRNA Ct value (mean±SD): Control = 7.62± 0.16), H2O2 = 7.47± 0.28. (E) Representative images of COL1, COL2, and ACAN immunocytochemistry of iAF cells treated with H2O2. (F) Quantification of immunocytochemistry in E, presented as percentage of total cells stained. Scale bars = 100μm. p S4 Fig.