Changes in domain-specific neutralization sensitivities of subtype B HIV-1 Envs over time.

For each neutralizing antibody (NAb), the geometric mean (GM) IC50 and percentage of pseudoviruses (PSVs) neutralized, was determined for all PSVs from each decade period, as shown in Fig 3. The trends for NAb potency (A-D) and breath (F-I) over time are shown by NAb specificity, including (A and F) V1V2 specific NAbs, (B and G) V3 NAbs, (C and H) CD4bs NAbs and (D and I) MPER NAbs. This neutralization analysis was repeated using 33 subtype B HIV+ plasma samples against PSVs from each decade period; the (E) GM ID50 and (J) percentage of PSVs neutralized, was determined for all PSVs from each decade. Significant differences were determined by Wilcoxon matched-pairs signed rank test; * = p<0.01, ** = p<0.001, *** = p<0.0001.