Algorithmic versus manual counting of EGFP⁺ neutrophils.

A) Violin plot showing similarity between distribution of manual and algorithmic neutrophil counts in 72 hpf Tg(lyz:EGFP) zebrafish expressing neutrophil-specific EGFP with highly similar mean values (N = 93 larvae). B) Orientation of anesthetized 72 hpf zebrafish (N = 192 larvae) plated in square 96-well plates suggesting that the potential discrepancy introduced by counting cells in fish in the non-lateral orientation is minimal because this sub-population accounts for such a small fraction of the whole.