The infection of follicle and nurse cells by <i>N</i>. <i>bombycis</i>.

(A-D) IFA showed that N. bombycis was infecting FC from ovariole sheath (A, B) and proliferated in FC (C, D). (E-F) TEM demonstrated the infection of FC by N. bombycis. (G-J) IFA displayed that N. bombycis infected NC from FC (G) and replicated in the NC (H-J). (K-L) TEM analysis of NCs infected by N. bombycis. The yellow dashed line indicates the boundary of FC and NC. Arrowhead, mature spores; arrow, proliferative N. bombycis; spores were stained using FB28 (blue); proliferative N. bombycis was labeled with anti-N. bombycis polyclonal antibody conjugated to Alexa Fluor 488 (green); Nucleus was stained using PI (red). N, nucleus; S, spore; ESP, empty spore shell; OS, ovariole sheath; FC, follicular cell; O, oocyte; NC, nurse cell.