<i>N</i>. <i>bombycis</i> infected oocyte from the follicle and nurse cells.

(A) IFA demonstrated the infection of oocyte by N. bombycis via the FC. (B) Observation of the uninfected FC and oocyte by TEM. (C-E) TEM showed the infection of oocyte by N. bombycis via the FC. (F) IFA illustrated the infection of oocyte by N. bombycis from the NC. (G) Observation of the uninfected NC and oocyte by TEM. (H, I) TEM displayed the infection of oocyte by N. bombycis from the NC. (J) N. bombycis in oocyte locates among the yolk granules. The arrow indicates the N. bombycis; spores were stained using FB28 (blue); the yellow dashed lines indicate the boundaries of cells; the red arrows indicate the direction in which the parasites enter the oocyte from FC or NC. N. bombycis in proliferation was labeled with anti-N. bombycis polyclonal antibody conjugated with Alexa Fluor 488 (green); Nuclei were stained by PI (red). N, Nucleus; S, spore; OS, ovariole sheath; FC, follicular cell; O, oocyte; NC, nurse cell; S, N. bombycis spores; Y, yolk granule.