SG-positive cells do not show MAVS or RIG-I localization in SG during SeV cbVG-high infection.

(A) SG (G3BP1, magenta) and MAVS (yellow) staining in A549 cells 24 hpi with RSV cbVG-high virus MOI 1.5 TCID50/cell. Zoomed in images of SG-positive cells are shown on the right with merge and MAVS and G3BP1 single channel. (B) SG (G3BP1, magenta) and RIG-I (yellow) staining in A549 cells 24 hpi with RSV cbVG-high virus MOI 1.5 TCID50/cell. Zoomed in images of SG-positive cells are shown on the right with merge and RIG-I and G3BP1 single channel. Widefield images at 40× magnification. cbVG, copy-back viral genome; G3BP1, GTPase-activating protein-binding protein 1; hpi, hours postinfection; MAVS, mitochondrial antiviral signaling; MOI, multiplicity of infection; RIG-I, retinoic acid–inducible gene I; RSV, respiratory syncytial virus; SeV, Sendai virus; SG, stress granule. (TIF)