Differences in SG formation in TMEV wt and ΔL infection.

(A-C) G3BP1, eIF3A and TIA1 are localized in aggresomes in DA virus-infected BHK-21 (A) and L929, but not HeLa cells (B). (D) G3BP1-positive SGs (arrows) and the aggresome (arrowhead) are rarely found in the cytoplasm of DAΔL-infected BHK-21 cells at 8 HPI. (E, F) Immunofluorescent staining of BHK-21 cells infected with DAΔL and GDVIIΔL virus at 8 HPI. (E) G3BP1-positive SGs are present in the cytoplasm of VP1-positive cells (arrows), while uninfected cells have homogeneous cytoplasmic G3BP1 staining. (F) SGs in GDVIIΔL virus-infected cells contain eIF3A and TIA1 (arrows). (G, H) Following infection with DAΔL and GDVIIΔL, TDP-43 and PTB1 are present in structures that resemble SGs (arrows); however, they infrequently colocalize with SG markers. Scale bars: 10 μm. *P P < 0.001.