Detection of MAYV RNA in NHP tissues at 10 dpi.

During necropsy extensive tissue subsets were collected from macaques and viral burden was determined through quantification of copies of viral RNA (vRNA) in qRT-PCR. Tissue subsets included muscles and joints (A), lymphoid tissues (B), genital and urinary (C), nervous system (D), and organs and glands (E). For all panels, the LOD was 500 copies per mL of tissue homogenate with undetectable samples graphed as 250 copies of vRNA/mL. Shown for each panel is a compilation and comparison of vRNA quantities for the tissue group, analyzed using a one-way ANOVA (ns = p > 0.05). All qRT-PCR reactions were performed in triplicate.