Study overview of MAYV infection of NHPs.

Schematic summarizing the MAYV macaque infection study (A). Pre-infection axillary, inguinal, and mesenteric lymph node and spleen biopsies as well as blood were collected one month prior to infection for three male rhesus macaques (RMs) ages 4, 10, and 13 years. Animals were inoculated with 105 plaque forming units (PFU) of MAYVBeAr505411 administered subcutaneously and spread evenly in both arms and hands. Blood was drawn for PBMC and plasma isolation as well as complete blood count (CBC) and serum chemistry at 0–5, 7, and 10 dpi. Animals were humanely euthanized at 10 dpi and extensive lymphoid, muscles, joints, nerves, lobes of the brain, heart, major organs, and male reproductive tissues were harvested. Plasma was isolated from blood collections at 0–5, 7, and 10 dpi for quantification of viral RNA in copies/mL of plasma by qRT-PCR in triplicate reactions (B). The qRT-PCR data is representative of three independent experiments. The LOD was 250 copies MAYV RNA per mL of plasma with undetectable samples graphed as 125 copies vRNA/mL plasma. Body temperatures (°F) (C) and change from starting body weight (%) (D) were recorded daily at all study timepoints.