Analysis of the sphere-forming capabilities of p53/BRCA1-deficient mammary tumor cells using the limiting dilution assay.

Limiting dilution analysis of TNF producing cells in C3H/HeJ mice.

E.C. Lattime
Antonella Stoppacciaro
O. Stutman

January 1988

A limiting dilution assay (LDA) that measures the frequency of TNF producing cells is described. LDA...

A mammary-specific model demonstrates the role of the p53 tumor suppressor gene in tumor development

Jerry, D. Joseph
Kittrell, F.S.
Kuperwasser, C.
Laucirica, R.
Dickinson, E.S.
Bonilla, P.J.
Butel, J.S.
Medina, D.

January 2000

Although alterations in the p53 tumor suppressor gene are detected frequently in human breast cancer...

Sphere formation assay.

Verena Tiran (3934577)
Stefanie Stanzer (3934574)
Ellen Heitzer (543730)
Michael Meilinger (3934565)
Christopher Rossmann (3934568)
Sigurd Lax (543733)
Oleksiy Tsybrovskyy (3934571)
Nadia Dandachi (3934559)
Marija Balic (3934562)

April 2017

Representative pictures from mammosphere assay. (A) PL11, left sphere from unsorted cells, middle...

Determining Mammosphere-Forming Potential: Application of the Limiting Dilution Analysis

Lauren M. Rota
Deborah A. Lazzarino
Amber N. Ziegler
Derek Leroith
Teresa L. Wood

January 2012

Abstract Originally adapted from the neurosphere assay, the nonadherent mammosphere assay has been u...

Tumor sphere/organoid-forming cells in p53/BRCA1-deficient mouse mammary tumors are largely FZD7+.

Aina He (357529)
Songhai Tian (6013694)
Oded Kopper (17364730)
Daniel J. Horan (17364733)
Peng Chen (6514)
Roderick T. Bronson (7386482)
Ren Sheng (7959113)
Hao Wu (65943)
Lufei Sui (17364736)
Kun Zhou (347672)
Liang Tao (248968)
Quan Wu (444646)
Yujing Huang (519658)
Zan Shen (280887)
Sen Han (2437435)
Xueqing Chen (499722)
Hong Chen (108084)
Xi He (208289)
Alexander G. Robling (8905064)
Rongsheng Jin (378622)
Hans Clevers (211819)
Dongxi Xiang (3693031)
Zhe Li (219258)
Min Dong (123378)

November 2023

(A) FACS analysis using FITC-TcdBFBD and anti-FZD7 antibody showed that primary p53/BRCA1-deficient ...

Tumorsphere number following 3 passages of p53/BRCA1-deficient mammary tumor cells with 150 nM TcdBFBD or TcdBmu or PBS in the sphere culture medium.

Aina He (357529)
Songhai Tian (6013694)
Oded Kopper (17364730)
Daniel J. Horan (17364733)
Peng Chen (6514)
Roderick T. Bronson (7386482)
Ren Sheng (7959113)
Hao Wu (65943)
Lufei Sui (17364736)
Kun Zhou (347672)
Liang Tao (248968)
Quan Wu (444646)
Yujing Huang (519658)
Zan Shen (280887)
Sen Han (2437435)
Xueqing Chen (499722)
Hong Chen (108084)
Xi He (208289)
Alexander G. Robling (8905064)
Rongsheng Jin (378622)
Hans Clevers (211819)
Dongxi Xiang (3693031)
Zhe Li (219258)
Min Dong (123378)

November 2023

Tumorsphere number following 3 passages of p53/BRCA1-deficient mammary tumor cells with 150 nM TcdBF...

Murine and Human Mammary Cancer Cell Lines: Functional Tests

Accornero, Paolo
Martignani, Eugenio
Miretti, Silvia
Baratta, Mario

January 2018

The biological characterization of mammary cancer cells is a prerequisite that helps the scientist u...

TcdBFBD inhibits tumorigenic potential of basal-like mammary tumor cells.

Aina He (357529)
Songhai Tian (6013694)
Oded Kopper (17364730)
Daniel J. Horan (17364733)
Peng Chen (6514)
Roderick T. Bronson (7386482)
Ren Sheng (7959113)
Hao Wu (65943)
Lufei Sui (17364736)
Kun Zhou (347672)
Liang Tao (248968)
Quan Wu (444646)
Yujing Huang (519658)
Zan Shen (280887)
Sen Han (2437435)
Xueqing Chen (499722)
Hong Chen (108084)
Xi He (208289)
Alexander G. Robling (8905064)
Rongsheng Jin (378622)
Hans Clevers (211819)
Dongxi Xiang (3693031)
Zhe Li (219258)
Min Dong (123378)

November 2023

(A) Cells dissociated from p53/BRCA1-deficient mammary tumor tissues were resuspended and plated int...

Crude analysis of mammary tumor development in SD, COP and (SD×COP)F1 strains.

Mayumi Nishimura (455516)
Kazuhiro Daino (203837)
Maki Fukuda (11272926)
Ikuya Tanaka (11272929)
Hitomi Moriyama (11272932)
Kaye Showler (11272935)
Yukiko Nishimura (11272938)
Masaru Takabatake (3202710)
Toshiaki Kokubo (11272941)
Atsuko Ishikawa (11272944)
Kazumasa Inoue (3701080)
Masahiro Fukushi (3202707)
Shizuko Kakinuma (763026)
Tatsuhiko Imaoka (455512)
Yoshiya Shimada (203843)

August 2021

Crude analysis of mammary tumor development in SD, COP and (SD×COP)F1 strains.</p

Cell proliferation assay for T47D and MDA-MB-231 breast cancer cell line and MCF-10A breast epithelial cell line.

Lu Chen (75170)
Lei Du-Cuny (682514)
Sylvestor Moses (682515)
Sabrina Dumas (682516)
Zuohe Song (682517)
Abdol Hossein Rezaeian (682518)
Hui-Kuan Lin (295008)
Emmanuelle J. Meuillet (682519)
Shuxing Zhang (682520)

January 2015

Cell proliferation assay for T47D and MDA-MB-231 breast cancer cell line and MCF-10A breast epith...

Summary of limiting dilution experiments of unsorted bulk tumor treated with TSA.

Terra Vleeshouwer-Neumann (833957)
Michael Phelps (833958)
Theo K. Bammler (128417)
James W. MacDonald (833959)
Isaac Jenkins (833960)
Eleanor Y. Chen (451826)

December 2015

Summary of limiting dilution experiments of unsorted bulk tumor treated with TSA.</p

Sphere formation assays and immunocytochemical analyses in tumor-initiating EpCAM+ cells treated with a p38 inhibitor (SB203580).

Tetsuhiro Chiba (508754)
Eiichiro Suzuki (508755)
Kaori Yuki (508756)
Yoh Zen (307071)
Motohiko Oshima (165144)
Satoru Miyagi (165159)
Atsunori Saraya (165151)
Shuhei Koide (508757)
Tenyu Motoyama (508758)
Sadahisa Ogasawara (508759)
Yoshihiko Ooka (508760)
Akinobu Tawada (508761)
Tetsuya Nakatsura (508762)
Takehiro Hayashi (508763)
Taro Yamashita (237137)
Syuichi Kaneko (508764)
Masaru Miyazaki (508765)
Atsushi Iwama (46665)
Osamu Yokosuka (234198)

January 2014

(A) Bright–field images of non-adherent spheres on day 14 of culture. Scale bar = 100 μm. (B) Num...

The percentage of FZD7+ cells are assessed in 3 breast cancer models.

Aina He (357529)
Songhai Tian (6013694)
Oded Kopper (17364730)
Daniel J. Horan (17364733)
Peng Chen (6514)
Roderick T. Bronson (7386482)
Ren Sheng (7959113)
Hao Wu (65943)
Lufei Sui (17364736)
Kun Zhou (347672)
Liang Tao (248968)
Quan Wu (444646)
Yujing Huang (519658)
Zan Shen (280887)
Sen Han (2437435)
Xueqing Chen (499722)
Hong Chen (108084)
Xi He (208289)
Alexander G. Robling (8905064)
Rongsheng Jin (378622)
Hans Clevers (211819)
Dongxi Xiang (3693031)
Zhe Li (219258)
Min Dong (123378)

November 2023

(A) Schematic diagram showing the generation of a p53/BRAC1-deficient breast cancer model by intradu...

Regulation of the p53 tumor suppressor gene in the mammary gland and its role in tumorigenesis

Kuperwasser, Charlotte

January 2000

Breast cancer is the most frequent tumor type among women. Heightened susceptibility of the breast t...

Evaluation of proliferation rate of TNBC cells (MDA-MB-231) and luminal-like breast cancer cells (MCF7).

Francesca Vitali (2490256)
Laurie D. Cohen (409381)
Andrea Demartini (3122754)
Angela Amato (84239)
Vincenzo Eterno (3122757)
Alberto Zambelli (10715)
Riccardo Bellazzi (74298)

September 2016

Evaluation of proliferation rate of TNBC cells (MDA-MB-231) and luminal-like breast cancer cells ...

Limiting dilution analysis of TNF producing cells in C3H/HeJ mice.

E.C. Lattime
Antonella Stoppacciaro
O. Stutman

January 1988

A limiting dilution assay (LDA) that measures the frequency of TNF producing cells is described. LDA...

A mammary-specific model demonstrates the role of the p53 tumor suppressor gene in tumor development

Jerry, D. Joseph
Kittrell, F.S.
Kuperwasser, C.
Laucirica, R.
Dickinson, E.S.
Bonilla, P.J.
Butel, J.S.
Medina, D.

January 2000

Although alterations in the p53 tumor suppressor gene are detected frequently in human breast cancer...

Sphere formation assay.

Verena Tiran (3934577)
Stefanie Stanzer (3934574)
Ellen Heitzer (543730)
Michael Meilinger (3934565)
Christopher Rossmann (3934568)
Sigurd Lax (543733)
Oleksiy Tsybrovskyy (3934571)
Nadia Dandachi (3934559)
Marija Balic (3934562)

April 2017

Representative pictures from mammosphere assay. (A) PL11, left sphere from unsorted cells, middle...

Determining Mammosphere-Forming Potential: Application of the Limiting Dilution Analysis

Lauren M. Rota
Deborah A. Lazzarino
Amber N. Ziegler
Derek Leroith
Teresa L. Wood

January 2012

Abstract Originally adapted from the neurosphere assay, the nonadherent mammosphere assay has been u...

Tumor sphere/organoid-forming cells in p53/BRCA1-deficient mouse mammary tumors are largely FZD7+.

Aina He (357529)
Songhai Tian (6013694)
Oded Kopper (17364730)
Daniel J. Horan (17364733)
Peng Chen (6514)
Roderick T. Bronson (7386482)
Ren Sheng (7959113)
Hao Wu (65943)
Lufei Sui (17364736)
Kun Zhou (347672)
Liang Tao (248968)
Quan Wu (444646)
Yujing Huang (519658)
Zan Shen (280887)
Sen Han (2437435)
Xueqing Chen (499722)
Hong Chen (108084)
Xi He (208289)
Alexander G. Robling (8905064)
Rongsheng Jin (378622)
Hans Clevers (211819)
Dongxi Xiang (3693031)
Zhe Li (219258)
Min Dong (123378)

November 2023

(A) FACS analysis using FITC-TcdBFBD and anti-FZD7 antibody showed that primary p53/BRCA1-deficient ...

Tumorsphere number following 3 passages of p53/BRCA1-deficient mammary tumor cells with 150 nM TcdBFBD or TcdBmu or PBS in the sphere culture medium.

Aina He (357529)
Songhai Tian (6013694)
Oded Kopper (17364730)
Daniel J. Horan (17364733)
Peng Chen (6514)
Roderick T. Bronson (7386482)
Ren Sheng (7959113)
Hao Wu (65943)
Lufei Sui (17364736)
Kun Zhou (347672)
Liang Tao (248968)
Quan Wu (444646)
Yujing Huang (519658)
Zan Shen (280887)
Sen Han (2437435)
Xueqing Chen (499722)
Hong Chen (108084)
Xi He (208289)
Alexander G. Robling (8905064)
Rongsheng Jin (378622)
Hans Clevers (211819)
Dongxi Xiang (3693031)
Zhe Li (219258)
Min Dong (123378)

November 2023

Tumorsphere number following 3 passages of p53/BRCA1-deficient mammary tumor cells with 150 nM TcdBF...

Murine and Human Mammary Cancer Cell Lines: Functional Tests

Accornero, Paolo
Martignani, Eugenio
Miretti, Silvia
Baratta, Mario

January 2018

The biological characterization of mammary cancer cells is a prerequisite that helps the scientist u...

TcdBFBD inhibits tumorigenic potential of basal-like mammary tumor cells.

Aina He (357529)
Songhai Tian (6013694)
Oded Kopper (17364730)
Daniel J. Horan (17364733)
Peng Chen (6514)
Roderick T. Bronson (7386482)
Ren Sheng (7959113)
Hao Wu (65943)
Lufei Sui (17364736)
Kun Zhou (347672)
Liang Tao (248968)
Quan Wu (444646)
Yujing Huang (519658)
Zan Shen (280887)
Sen Han (2437435)
Xueqing Chen (499722)
Hong Chen (108084)
Xi He (208289)
Alexander G. Robling (8905064)
Rongsheng Jin (378622)
Hans Clevers (211819)
Dongxi Xiang (3693031)
Zhe Li (219258)
Min Dong (123378)

November 2023

(A) Cells dissociated from p53/BRCA1-deficient mammary tumor tissues were resuspended and plated int...

Crude analysis of mammary tumor development in SD, COP and (SD×COP)F1 strains.

Mayumi Nishimura (455516)
Kazuhiro Daino (203837)
Maki Fukuda (11272926)
Ikuya Tanaka (11272929)
Hitomi Moriyama (11272932)
Kaye Showler (11272935)
Yukiko Nishimura (11272938)
Masaru Takabatake (3202710)
Toshiaki Kokubo (11272941)
Atsuko Ishikawa (11272944)
Kazumasa Inoue (3701080)
Masahiro Fukushi (3202707)
Shizuko Kakinuma (763026)
Tatsuhiko Imaoka (455512)
Yoshiya Shimada (203843)

August 2021

Crude analysis of mammary tumor development in SD, COP and (SD×COP)F1 strains.</p

Cell proliferation assay for T47D and MDA-MB-231 breast cancer cell line and MCF-10A breast epithelial cell line.

Lu Chen (75170)
Lei Du-Cuny (682514)
Sylvestor Moses (682515)
Sabrina Dumas (682516)
Zuohe Song (682517)
Abdol Hossein Rezaeian (682518)
Hui-Kuan Lin (295008)
Emmanuelle J. Meuillet (682519)
Shuxing Zhang (682520)

January 2015

Cell proliferation assay for T47D and MDA-MB-231 breast cancer cell line and MCF-10A breast epith...

Summary of limiting dilution experiments of unsorted bulk tumor treated with TSA.

Terra Vleeshouwer-Neumann (833957)
Michael Phelps (833958)
Theo K. Bammler (128417)
James W. MacDonald (833959)
Isaac Jenkins (833960)
Eleanor Y. Chen (451826)

December 2015

Summary of limiting dilution experiments of unsorted bulk tumor treated with TSA.</p

Sphere formation assays and immunocytochemical analyses in tumor-initiating EpCAM+ cells treated with a p38 inhibitor (SB203580).

Tetsuhiro Chiba (508754)
Eiichiro Suzuki (508755)
Kaori Yuki (508756)
Yoh Zen (307071)
Motohiko Oshima (165144)
Satoru Miyagi (165159)
Atsunori Saraya (165151)
Shuhei Koide (508757)
Tenyu Motoyama (508758)
Sadahisa Ogasawara (508759)
Yoshihiko Ooka (508760)
Akinobu Tawada (508761)
Tetsuya Nakatsura (508762)
Takehiro Hayashi (508763)
Taro Yamashita (237137)
Syuichi Kaneko (508764)
Masaru Miyazaki (508765)
Atsushi Iwama (46665)
Osamu Yokosuka (234198)

January 2014

(A) Bright–field images of non-adherent spheres on day 14 of culture. Scale bar = 100 μm. (B) Num...

The percentage of FZD7+ cells are assessed in 3 breast cancer models.

Aina He (357529)
Songhai Tian (6013694)
Oded Kopper (17364730)
Daniel J. Horan (17364733)
Peng Chen (6514)
Roderick T. Bronson (7386482)
Ren Sheng (7959113)
Hao Wu (65943)
Lufei Sui (17364736)
Kun Zhou (347672)
Liang Tao (248968)
Quan Wu (444646)
Yujing Huang (519658)
Zan Shen (280887)
Sen Han (2437435)
Xueqing Chen (499722)
Hong Chen (108084)
Xi He (208289)
Alexander G. Robling (8905064)
Rongsheng Jin (378622)
Hans Clevers (211819)
Dongxi Xiang (3693031)
Zhe Li (219258)
Min Dong (123378)

November 2023

(A) Schematic diagram showing the generation of a p53/BRAC1-deficient breast cancer model by intradu...

Regulation of the p53 tumor suppressor gene in the mammary gland and its role in tumorigenesis

Kuperwasser, Charlotte

January 2000

Breast cancer is the most frequent tumor type among women. Heightened susceptibility of the breast t...

Evaluation of proliferation rate of TNBC cells (MDA-MB-231) and luminal-like breast cancer cells (MCF7).

Francesca Vitali (2490256)
Laurie D. Cohen (409381)
Andrea Demartini (3122754)
Angela Amato (84239)
Vincenzo Eterno (3122757)
Alberto Zambelli (10715)
Riccardo Bellazzi (74298)

September 2016

Evaluation of proliferation rate of TNBC cells (MDA-MB-231) and luminal-like breast cancer cells ...

Limiting dilution analysis of TNF producing cells in C3H/HeJ mice.

E.C. Lattime
Antonella Stoppacciaro
O. Stutman

January 1988

A limiting dilution assay (LDA) that measures the frequency of TNF producing cells is described. LDA...

A mammary-specific model demonstrates the role of the p53 tumor suppressor gene in tumor development

Jerry, D. Joseph
Kittrell, F.S.
Kuperwasser, C.
Laucirica, R.
Dickinson, E.S.
Bonilla, P.J.
Butel, J.S.
Medina, D.

January 2000

Although alterations in the p53 tumor suppressor gene are detected frequently in human breast cancer...

Sphere formation assay.

Verena Tiran (3934577)
Stefanie Stanzer (3934574)
Ellen Heitzer (543730)
Michael Meilinger (3934565)
Christopher Rossmann (3934568)
Sigurd Lax (543733)
Oleksiy Tsybrovskyy (3934571)
Nadia Dandachi (3934559)
Marija Balic (3934562)

April 2017

Representative pictures from mammosphere assay. (A) PL11, left sphere from unsorted cells, middle...

Analysis of the sphere-forming capabilities of p53/BRCA1-deficient mammary tumor cells using the limiting dilution assay.

Abstract

Extracted data

Analysis of the sphere-forming capabilities of p53/BRCA1-deficient mammary tumor cells using the limiting dilution assay.

Abstract

Extracted data

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