Genome-scale metabolic modelling of male subjects.

A) Clustering of context-specific models. Each column represents a reaction in the human metabolic network and its color accounts for the (normalized) activity of that specific reaction in the corresponding context-specific model. Models are clustered according to their profile of active/inactive reactions. B) Heatmap that includes those metabolic processes (subsystems) showing an overall flux increase that paralleled that of disease severity (from mild to severe). Their activity in the healthy-model is also included in the figure for clarity. C) Fold change of average metabolic activity for each subsystem in the healthy vs. fatal condition. D) Heatmaps showing the clustering of the different organs according to the activity of each reaction (left healthy state, right fatal state). As in in the panel A, each column represents a reaction in the human metabolic network and its color accounts for the (normalized) activity of that specific reaction in the corresponding organ. In the heatmap on the left, three main clusters are labelled with a red, green and orange dot and the organs belonging to each cluster are labelled accordingly and this color code is maintained in the heatmap on the right. Organs changing clusters in the two heatmaps are connected with a line.