Inverse modelling approach.

(A) Network C57BL/6I, where red edges represent SAs with in vivo RBC velocity measurements. Furthermore, purple and blue dots highlight the locations of DA and AV roots, where a range for the RBC velocities is prescribed. (B) Illustration of the two types of cost functions used in this work for prescribing target values (left) or ranges (right) for the RBC velocities. (C) RBC velocities in SAs, DAs and AVs before and after tuning. (D-E) Diameters (D) and RBC velocities (E) at the SAs after tuning the semi-realistic network (see also S11 Fig).