A high-resolution record of exceptionally well preserved calcareous nannofossil assemblages from Tanzania is marked by two key transitions closely related to the climatic events of the Eocene-Oligocene transition (EOT). The first transition, at ∼34.0 Ma, precedes the first positive shift in δ 18O and coincides with a distinct interval of very low nannofossil abundance and a cooling in sea surface temperatures (SST). The second, at ∼33.63 Ma, is immediately above the Eocene-Oligocene boundary (EOB) and is associated with a significant drop in nannofossil diversity. Both transitions involve significant reductions in the abundance of holococcoliths and other oligotrophic taxa. These changes in calcareous phytoplankton assemblages indicate (1) ...