Cryo-EM imaging of vitreous samples is limited to a few hundred nanometers in thickness. Focused ion beams can mill windows into cells and tissues for imaging, but they damage biological samples. In this issue of Structure, Yang et al. (2023) quantitatively describe this damage and suggest ways to minimize it
Progress in understanding the inner structure of cells, as well as the cell-matrix structures, is dr...
The development of cryo-focused ion beam (cryo-FIB) for the thinning of frozen-hydrated biological s...
Cryogenic electron microscopy and data processing enable the determination of structures of isolated...
Cryo-EM imaging of vitreous samples is limited to a few hundred nanometers in thickness. Focused ion...
As cryo-EM approaches the physical resolution limits imposed by electron optics and radiation damage...
The ability to precisely control the preparation of biological samples for investigations by electro...
Three-dimensional (3D) imaging is an important tool in electron microscopy, especially in biological...
Focused Ion Beam (FIB) devices such as the Helium Ion Microscope (HIM) as well as FIB/SEMs have spar...
Imaging of biomolecules by ionizing radiation, such as electrons, causes radiation damage which intr...
Cryo-electron tomography (CET) is a well-established technique for imaging cellular and molecular st...
Volume microscopy at high resolution is increasingly required to better understand cellular function...
Cryo-electron tomography (cryo-ET) has become the method of choice for investigating cellular ultras...
Electron cryotomography is able to visualize macromolecular complexes in their cellular context, in ...
While cryo-electron tomography (cryo-ET) can reveal biological structures in their native state with...
Fluorescence microscopy is a powerful tool for localising proteins within biological samp...
Progress in understanding the inner structure of cells, as well as the cell-matrix structures, is dr...
The development of cryo-focused ion beam (cryo-FIB) for the thinning of frozen-hydrated biological s...
Cryogenic electron microscopy and data processing enable the determination of structures of isolated...
Cryo-EM imaging of vitreous samples is limited to a few hundred nanometers in thickness. Focused ion...
As cryo-EM approaches the physical resolution limits imposed by electron optics and radiation damage...
The ability to precisely control the preparation of biological samples for investigations by electro...
Three-dimensional (3D) imaging is an important tool in electron microscopy, especially in biological...
Focused Ion Beam (FIB) devices such as the Helium Ion Microscope (HIM) as well as FIB/SEMs have spar...
Imaging of biomolecules by ionizing radiation, such as electrons, causes radiation damage which intr...
Cryo-electron tomography (CET) is a well-established technique for imaging cellular and molecular st...
Volume microscopy at high resolution is increasingly required to better understand cellular function...
Cryo-electron tomography (cryo-ET) has become the method of choice for investigating cellular ultras...
Electron cryotomography is able to visualize macromolecular complexes in their cellular context, in ...
While cryo-electron tomography (cryo-ET) can reveal biological structures in their native state with...
Fluorescence microscopy is a powerful tool for localising proteins within biological samp...
Progress in understanding the inner structure of cells, as well as the cell-matrix structures, is dr...
The development of cryo-focused ion beam (cryo-FIB) for the thinning of frozen-hydrated biological s...
Cryogenic electron microscopy and data processing enable the determination of structures of isolated...
DOI
Add to ORKG