Sex differences in BALF and lung tissue neutrophils, monocytes, T cells, and alveolar macrophages during allergic inflammation.

Quantification of neutrophils (A), monocytes (B), T cells (C), and alveolar macrophages (D) in bronchoalveolar lavages (left) and lung homogenates (right) of male and female mice by multi-color flow cytometry. Data represented as absolute cell counts. When comparing challenge time point responses (1–6) relative to baseline (for either male or female data) by two-way ANOVA: *p < 0.05; **p < 0.01; #p < 0.001; †p < 0.0001. Gray shaded boxes represent a statistically significant difference (p < 0.05) between males and females at that particular time-point. N = 4 mice of each sex per time point for “Baseline” and “Challenge 6” groups, N = 8 mice/sex per time point for “Challenge 1–5” groups. Data are from a single experiment representative of two independent experiments.