Sexual dimorphism in B cells, OVA-specific IgE, and serum OVA-specific IgG1 immunoglobulins.

(A) Quantification of B cells in bronchoalveolar lavage and lung tissue of male and female mice by multi-color flow cytometry. (B) Measurements of serum OVA-specific IgE and OVA-specific IgG1 during challenge phase of the model (inflammation). (C) Measurements of serum OVA-specific IgE and IgG1 following intraperitoneal antigen sensitization and prior to the first OVA challenge (sensitization). Two-way ANOVA: *p A. and B.: N = 4 mice of each sex per time point for “Baseline” and “Challenge 6” groups, N = 6 mice/sex per time point for “Challenge 1–2” groups, N = 8 mice/sex per time point for “Challenge 3–5” groups. Data are from a single experiment representative of at least two independent experiments. For graphs in C.: N = 4 mice/sex for “Saline” groups, N = 5 mice/sex for “Sensitization” groups. Data are from a single experiment.