(A and B) Solution behavior of native (A) or refolded (B) DENV3 MTase protein on a Superdex 200 Increase 10/300 GL column. The inset figure shows the SDS-PAGE analyses of the pooled samples. (C and D) Affinity determination between native (C) or refolded (D) DENV3 MTase and SAM by ITC. (TIF)</p
Lanes in panel A: 1, Mtb cytosol; 2, MtM; 3, MtM-SMALPs; 4, SMA-insoluble MtM and 5, lyophilized MtM...
<p>Interactions between enzymes and NADPH were detected by isothermal titration calorimetry using na...
<p>(<b>A</b>) Hydrophobic interaction FPLC chromatogram (absorbance at 214 nm) of the protein extrac...
Solution behaviors of DENV3 and ALSV mutant MTase proteins on a Superdex 200 Increase 10/300 GL colu...
(A) Binding of SAM to native or refolded DENV3 MTase mutants (H110A, E111A, E112A, H110A/E111A or H1...
(A) Binding of SAM to native or refolded DENV3 MTase mutants (H110A, E111A, E112A, H110A/E111A or H1...
(A) Binding of SAM to native or refolded DENV3 MTase mutants (H110A, E111A, E112A, H110A/E111A or H1...
(A) A schematic view of the protein-engineering strategy used to yield ALSV MTase. The putative sign...
<p>(<b>A</b>) Dose response of inhibition of the [<sup>3</sup>H]-SAM-MTase complex formation by AdoM...
<p>(A) Binding to a 420 base pair DNA fragment was measured by EMSAs for increasing concentrations o...
<p>The MTase was in cartoon representation in grey color with representative contact residues in sti...
<p>Predicted poses of compounds 306711 and 610920 in the DENV3 MTase SAM-binding pocket. <b>(A)</b> ...
<p>A) The WT and mutant enzymes are shown above the designated lane. Approximately 5 µg of each prot...
(a) Schematic diagram of the construct used for comparing the GFP trap and the ligand pull-down meth...
ITC titration data for the nsp14/nsp10 complex for SAM (left), SGC0946 (center), and SGC8158 (right)...
Lanes in panel A: 1, Mtb cytosol; 2, MtM; 3, MtM-SMALPs; 4, SMA-insoluble MtM and 5, lyophilized MtM...
<p>Interactions between enzymes and NADPH were detected by isothermal titration calorimetry using na...
<p>(<b>A</b>) Hydrophobic interaction FPLC chromatogram (absorbance at 214 nm) of the protein extrac...
Solution behaviors of DENV3 and ALSV mutant MTase proteins on a Superdex 200 Increase 10/300 GL colu...
(A) Binding of SAM to native or refolded DENV3 MTase mutants (H110A, E111A, E112A, H110A/E111A or H1...
(A) Binding of SAM to native or refolded DENV3 MTase mutants (H110A, E111A, E112A, H110A/E111A or H1...
(A) Binding of SAM to native or refolded DENV3 MTase mutants (H110A, E111A, E112A, H110A/E111A or H1...
(A) A schematic view of the protein-engineering strategy used to yield ALSV MTase. The putative sign...
<p>(<b>A</b>) Dose response of inhibition of the [<sup>3</sup>H]-SAM-MTase complex formation by AdoM...
<p>(A) Binding to a 420 base pair DNA fragment was measured by EMSAs for increasing concentrations o...
<p>The MTase was in cartoon representation in grey color with representative contact residues in sti...
<p>Predicted poses of compounds 306711 and 610920 in the DENV3 MTase SAM-binding pocket. <b>(A)</b> ...
<p>A) The WT and mutant enzymes are shown above the designated lane. Approximately 5 µg of each prot...
(a) Schematic diagram of the construct used for comparing the GFP trap and the ligand pull-down meth...
ITC titration data for the nsp14/nsp10 complex for SAM (left), SGC0946 (center), and SGC8158 (right)...
Lanes in panel A: 1, Mtb cytosol; 2, MtM; 3, MtM-SMALPs; 4, SMA-insoluble MtM and 5, lyophilized MtM...
<p>Interactions between enzymes and NADPH were detected by isothermal titration calorimetry using na...
<p>(<b>A</b>) Hydrophobic interaction FPLC chromatogram (absorbance at 214 nm) of the protein extrac...