<i>gcn4</i>∆ cells accumulate less storage carbohydrates and produce less ethanol.

Cells were grown in minimal medium without amino acids. A) Relative percentage of glycogen and trehalose contents in gcn4∆ cells with respect to wild-type cells at stationary phase (72 hours). B: Glucose concentration was determined in the culture medium of wild-type and gcn4∆ cells at exponential growth phase (18 hours). Glucose consumption was calculated as the difference between the initial glucose concentration and the concentration in each tested condition. Values indicate the average of at least 3 independent experiments and the deviation indicates the standard error of the mean (SEM). The two-way ANOVA test was performed. C: Ethanol concentration (mM) was determined in the culture medium of wild-type and gcn4∆ cells at exponential growth phase (18 hours). Values indicate the average of at least 3 independent experiments and the deviation indicates the standard error of the mean (SEM). The two-way ANOVA test (****: p<0.0001) was performed.