Yeast cells lacking <i>BXI1</i> are more susceptible to ethanol-induced and glucose-induced programmed cell death.

<p>Wildtype and <i>Δbxi1</i> cells from the BY4742 strain background were cultured in rich YPD media overnight and resuspended in fresh media and allowed to reach exponential phase (an approximate OD₆₀₀ value of 0.4). (A) Exponential growing yeast cells were then resuspended in fresh media or in fresh media containing 22% ethanol, allowed to grow at 30°C for the indicated times, serially diluted onto YPD plates, and cultured at 30°C for 2 days. (B) Exponential growing yeast cells were then resuspended in water or in 2% glucose, allowed to grow at 30°C for the indicated times, serially diluted onto YPD plates, and cultured at 30°C for 2 days. The differences in viabilities were deemed statistically significant by the Student's t-test (p<0.05). Error bars indicate standard deviations for trials with at least three independent cultures.