Yeast cells lacking <i>BXI1</i> are sensitive to β-mercaptoethanol and tunicamycin.

<p>Wildtype and <i>Δbxi1</i> cells from the BY4742 and Σ1278b strain backgrounds were cultured in rich YPD media overnight. They were then resuspended in fresh media and allowed to reach exponential phase (an approximate OD₆₀₀ value of 0.4). For each strain, a series of 10-fold dilutions was then prepared in water over a range of concentrations from 10⁻¹ to 10⁻⁵ relative to the initial culture. Spots of 5 µl from each dilution series were then plated on the indicated media and cultured at 30°C for 2 days. Plates supplemented with drugs were poured and used on the same day. All spot assays were repeated at least three times and a representative experiment is shown.