Energy consumption and water-soluble protein release by cell wall disruption of Nannochloropsis gaditana.
- L. Cabas Rodriguez
- Lolke Sijtsma
- N. Engelen-Smit
- Giuseppe Olivieri
- W. Spekking
- W.J. Mulder
- L.A.M. van den Broek
- Carl Safi
DOIAbstract
Several cell disruption methods were tested on Nannochloropsis gaditana, to evaluate their efficiency in terms of cell disintegration, energy input and release of soluble proteins. High-pressure homogenization (HPH) and bead milling were the most efficient with >95% cell disintegration, ±50% (w/w) release of total proteins and low energy input (<0.5 kWh.kg−1 biomass). Enzymatic treatment required low energy input (<0.34 kWh.kg−1 biomass), but it only released ±35% protein (w/w). Pulsed Electric Field (PEF) was neither energy-efficient (10.44 kWh.kg−1 biomass) nor successful for protein release (only 10% proteins w/w) and cell disintegration. The release of proteins after applying HPH and bead milling always required less intensive operating...
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