TcIPCS1 model prediction and topology.

(A) Rosetta prediction of TcIPCS Esmeraldo-like allele (TcCLB.506885.124). Color palette indicates per-residue confidence score (pLDDT), which ranges from 0 to 100. (B) Topology diagram (schematic) of TcIPCS. (C) Key residues for the catalytic triad (H202, H245, D249) and substrate selectivity (S244). Predicted location of the TcIPCS active site with residues and loops are indicated. (D) Quantitative RT-PCR analysis of the TcIPCS transcript levels obtained from RNA isolated from amastigotes and tissue culture derived trypomastigote stages as well as epimastigote cultures. Statistical significance was determined by one-way ANOVA (n = 5; ***, P < 0.001).