Comparison of the crystal structures of CD64 with density plots of the 100 best-fit models of CD64.

(A-D) Ribbon diagrams of the four CD64 crystal structures correspond to unbound CD64 (PDB code 3RJD) and the three CD64-Fc co-crystals (PDB codes B, 4W4O, C, 4X4M and D, 4ZNE). The glycans in the crystal structures are shown as yellow sticks. (E-H) The density plots of the four sets of putative 100 best-fit CD64 models at four concentrations are shown. The energy-minimised starting structure of CD64 is shown as a black cartoon in the same views in all four panels. The 100 best-fit models were superimposed on the D2 domain. The volumes occupied by the D1, D2 and D3 domains in the 100 best-fit structures are represented as blue, orange and red wireframes respectively in each density plot. Two different views rotated by 90° of each density plot are displayed. In panel E, we also show a stick representation of 10 of the 100 best-fit CD64 models in a smaller scale to clarify how the density plots related to the atomistic models, with the best fit model shown as a blue ribbon.