ROR2 expression and distribution in ependymal cells.

(A-C) Immunohistochemical staining of SCO-Spondin and ROR2 in frontal brain sections containing SCO cells and ependymal cells from normoglycemic and hyperglycemic animals. Scale bar: 20 μm. ROR2 was not expressed in SCO cells. Under normoglycemic conditions, ROR2 showed mostly focal immunoreactivity and was internalized in ependymal cells (immunofluorescence and DIC images) (A). However, under hyperglycemic conditions, ROR2 immunoreactivity was detected mainly in the apical membrane of ependymal cells and intracellularly (B and C, areas 1–3). Scale bar: 20 μm. CSF, cerebrospinal fluid; DIC, differential interference contrast; d3v, dorsal third ventricle; ROR2, Frizzled 2/receptor tyrosine kinase-like orphan receptor-2; SCO, subcommissural organ. (TIF)