CC071 MEFs fail to control viral replication, with delayed <i>Ifnb1</i> expression but normal response to IFN-I.

(A, B) MEFs derived from B6 (black circles), CC001 (blue triangles) and CC071 (red squares) were infected with ZIKV at a MOI of 5 and analyzed 24, 48 and 72 hpi. (A) Viral titer in supernatants was quantified by FFA. (B) Ifnb1 expression was normalized to Tbp reference gene. Data are mean +/- sem from 3 to 4 biological replicates per strain (MEFs derived from individual embryos). (C) MEFs were stimulated with recombinant IFNα. Ifitm3 relative expression normalized to Tbp reference gene is shown as an example of ISG. Data are mean +/- sem from 2 to 3 biological replicates. Blue asterisks and black hashes show statistical significance of CC071 compared to CC001 and to B6, respectively (ANOVA followed by Tukey HSD, */# p < 0.05, **/## p < 0.01, ***/### p < 0.001).