RIV, SMB, and SAA neurons influence reversal duration.

Neurons RIV, SMB, and SAA were optogenetically inhibited when worms spontaneously reversed. The time spent going backwards is reported in a 10-s window coinciding with optogenetic inhibition upon reversal onset. Worms expressed the inhibitory opsin gtACR2 in neurons RIV, SMB, and SAA under the lim-4 promoter. Illumination intensity of either 180 μW/mm2 (“+”) or 2 μW/mm2 (“o” control) was delivered. Worms spent more time reversing when these neurons were inhibited than in the control. Error bars represent 95% confidence intervals; p value via 2 proportion Z-test is 1.93E−09. N = 612 and 695 stimulus events for “o” and “+” conditions, respectively, across 14 plates. All data underlying this figure can be found at https://doi.org/10.25452/figshare.plus.23903202.