The implementation of APK pathway in vivo.

(A) Schematic representation of the natural glycerol metabolic pathways and APK pathway. Red crosses represent gene knockout. Blue arrows represent APK pathway. The promoter Ptac was used to overexpress pk in the plasmid. Metabolite abbreviation: DHA, dihydroxyacetone; DHAP, dihydroxyacetone phosphate; AcP, acetyl phosphate. Genes involved: glpk, glycerol kinase; dhak, dihydroxyacetone kinase. (B) Growth curve in glycerol minimal medium. The control was MG1655ΔglpkΔdhak. The strain PK (SPK) was MG1655ΔglpkΔdhak harboring plasmid pBD-PK. Plasmid pBD-PK was constructed for the expression of pk under the control of the Ptac promoter. Error bars represent SD (standard deviation), n = 3. The raw data was listed in S1 Data. (TIF)