The Effect of Chayote Juice (Sechium Edule) on Mda and Il-6 Levels (Experimental Study on Male Wistar Rats Induced with High-Fat Diet)

Dyslipidemia is an increase in total cholesterol, Low-Density Lipoprotein (LDL), triglycerides, and a decrease high- Density Lipoprotein (HDL), Consequences of consuming fat cause the metabolism of free fatty acids resulting in an imbalance between free radicals and the body's natural antioxidants which causes a condition of oxidative stress. Oxidative stress results in tissue damage which triggers an inflammatory reaction that releases pro-inflammatory cytokines such as Interleukin-6 (IL-6) and triggers Malondialdehyde (MDA) due to lipid oxidation in tissues. Consuming antioxidants from outside the body can neutralize this condition, such as chayote juice. The aim of this study is to know the effect of giving chayote juice on the rate of MDA and IL- 6 in male Wistar rats who are given a high-fat diet. The research method is experimental research with a post-test-only control group design. The study subjects were 24 male Wistar rats, randomly divided into 4 groups. Group K1 was given standard feed and distillate water. Group K2 was without chayote juice but was given a high-fat diet. Groups- K3 and K4 were given a high-fat diet and chayote juice at doses of 1.8mL/200gBW/day and 3.6mL/200gBW/day, respectively. The study was conducted at IBL FK UNISSULA for 29 days by taking blood to check MDA levels using the TBARS and IL-6 methods using the ELISA method. Results showed that the lowest average MDA level was in the K4 group (0.058 ng/L) and the lowest average IL-6 level was in the K3 group (4.513 ppm). One-way ANOVA test result showed a significant difference in MDA levels with p = 0.021 and IL-6 levels showed no significant difference with p = 0.108. The Tahmane test result showed the MDA levels in K1 and K4 showed a significant difference in K2 (p<0.05). Test LSD test result showed the IL-6 levels in K3 showed a significant difference to K2 (p