Fig. 3. Products (Me/TMS) of 9(S)-HPOD (A and B) and 13(S)-HPOD (C and D) conversions by recombinant WT LeAOS3 (А and C) and LeAOS F108L mutant form (B and D). 1a and 1b, threo and erythro isomers of 9,10-dihydroxy-12- octadecenoic acid; 3, 10-hydroxyphytonoic acid; 4, 9,10-epoxy-11-hydroxy-12- octadecenoic acid; 6a and 6b, threo and erythro isomers of 12,13-dihydroxy-9- octadecenoic acid; 8, 11-hydroxy-12,13-epoxy-9-octadecenoic acid; 10, 9-hydroxy-12,13-epoxy-10-octadecenoic acid. The structural formulae of products are presented in Fig. 2. 9-HOD, (9S,10E,12Z)-9-hydroxy-10,12-octadecadienoic acid.Published as part of Toporkova, Yana Y., Smirnova, Elena O., Mukhtarova, Lucia S., Gorina, Svetlana S. & Grechkin, Alexander N., 2020, Catalysis...
Fig. 3. The total ion current (TIC) GC–MS chromatograms of products (Me/TMS) of incubations of the r...
AbstractBioinformatics analyses enabled us to identify the hypothetical determinants of catalysis by...
Fig. 2. The structural formulae of products of recombinant enzyme incubations with different substra...
Fig. 5. GC-MS analyses of products (Me/TMS) of 9(S)-HPOD conversions by recombinant WT ZmAOS1 (А), P...
Fig. 4. GC-MS analyses of products (Me/TMS) of 9(S)-HPOT conversions by recombinant WT ZmAOS1 (А), P...
Fig. 2. Structural formulae of reaction products (Me/ TMS) of target WT enzymes and their mutant for...
Fig. 6. The mechanism of fatty acid hydroperoxide conversion by CYP74 proteins showing the role of t...
Toporkova, Yana Y., Smirnova, Elena O., Mukhtarova, Lucia S., Gorina, Svetlana S., Grechkin, Alexand...
Fig. 1. Multiple alignments of partial sequences of following CYP74s: The multiple alignment of diff...
Fig. 5. GC-MS analyses of products (Me/TMS) of LuDES F291V mutant form incubations with 9(S)-HPOD (A...
Fig. 3. GC-MS analyses of products (Me/TMS) of recombinant NtDES incubations with 9(S)-HPOD (A), 9(S...
Fig. 2. Structural formulae of reaction products of target WT enzymes and their mutant forms. 1, col...
Fig. 4. GC-MS analyses of products (Me/TMS) of recombinant WT LuDES incubations with 9(S)-HPOD (A), ...
AbstractNon-classical P450s of CYP74 family control several enzymatic conversions of fatty acid hydr...
Fig. 4. The TIC GC–MS chromatograms of products (Me/TMS) of incubations of the recombinant CYP74L2 w...
Fig. 3. The total ion current (TIC) GC–MS chromatograms of products (Me/TMS) of incubations of the r...
AbstractBioinformatics analyses enabled us to identify the hypothetical determinants of catalysis by...
Fig. 2. The structural formulae of products of recombinant enzyme incubations with different substra...
Fig. 5. GC-MS analyses of products (Me/TMS) of 9(S)-HPOD conversions by recombinant WT ZmAOS1 (А), P...
Fig. 4. GC-MS analyses of products (Me/TMS) of 9(S)-HPOT conversions by recombinant WT ZmAOS1 (А), P...
Fig. 2. Structural formulae of reaction products (Me/ TMS) of target WT enzymes and their mutant for...
Fig. 6. The mechanism of fatty acid hydroperoxide conversion by CYP74 proteins showing the role of t...
Toporkova, Yana Y., Smirnova, Elena O., Mukhtarova, Lucia S., Gorina, Svetlana S., Grechkin, Alexand...
Fig. 1. Multiple alignments of partial sequences of following CYP74s: The multiple alignment of diff...
Fig. 5. GC-MS analyses of products (Me/TMS) of LuDES F291V mutant form incubations with 9(S)-HPOD (A...
Fig. 3. GC-MS analyses of products (Me/TMS) of recombinant NtDES incubations with 9(S)-HPOD (A), 9(S...
Fig. 2. Structural formulae of reaction products of target WT enzymes and their mutant forms. 1, col...
Fig. 4. GC-MS analyses of products (Me/TMS) of recombinant WT LuDES incubations with 9(S)-HPOD (A), ...
AbstractNon-classical P450s of CYP74 family control several enzymatic conversions of fatty acid hydr...
Fig. 4. The TIC GC–MS chromatograms of products (Me/TMS) of incubations of the recombinant CYP74L2 w...
Fig. 3. The total ion current (TIC) GC–MS chromatograms of products (Me/TMS) of incubations of the r...
AbstractBioinformatics analyses enabled us to identify the hypothetical determinants of catalysis by...
Fig. 2. The structural formulae of products of recombinant enzyme incubations with different substra...