Fig. 5. GC-MS analyses of products (Me/TMS) of 9(S)-HPOD conversions by recombinant WT ZmAOS1 (А), PpAOS2 F93L (B), and ZmAOS1 F95L (C). 1a and 1b, threo and erythro isomers of 9,10-dihydroxy-12-octadecenoic acid; 4, 9,10-epoxy-11-hydroxy-12-octadecenoic acid; 12, 9,10-epoxy-13-hydroxy-11- octadecenoic acid; 13, 9-hydroxynonanoic acid. The structural formulae of products are presented in Fig. 2. 9-HOD, (9S,10E,12Z)-9-hydroxy-10,12-octadecadienoic acid.Published as part of Toporkova, Yana Y., Smirnova, Elena O., Mukhtarova, Lucia S., Gorina, Svetlana S. & Grechkin, Alexander N., 2020, Catalysis by allene oxide synthases (CYP74A and CYP74C): Alterations by the Phe/Leu mutation at the SRS-1 region, pp. 1-9 in Phytochemistry (112152) 169 on pag...
Fig. 3. The total ion current (TIC) GC–MS chromatograms of products (Me/TMS) of incubations of the r...
AbstractBioinformatics analyses enabled us to identify the hypothetical determinants of catalysis by...
The product specificity and mechanistic peculiarities of two allene oxide synthases, tomato LeAOS3 (...
Fig. 4. GC-MS analyses of products (Me/TMS) of 9(S)-HPOT conversions by recombinant WT ZmAOS1 (А), P...
Fig. 3. Products (Me/TMS) of 9(S)-HPOD (A and B) and 13(S)-HPOD (C and D) conversions by recombinant...
Fig. 2. Structural formulae of reaction products (Me/ TMS) of target WT enzymes and their mutant for...
Fig. 6. The mechanism of fatty acid hydroperoxide conversion by CYP74 proteins showing the role of t...
Toporkova, Yana Y., Smirnova, Elena O., Mukhtarova, Lucia S., Gorina, Svetlana S., Grechkin, Alexand...
Fig. 1. Multiple alignments of partial sequences of following CYP74s: The multiple alignment of diff...
Fig. 5. GC-MS analyses of products (Me/TMS) of LuDES F291V mutant form incubations with 9(S)-HPOD (A...
Fig. 4. GC-MS analyses of products (Me/TMS) of recombinant WT LuDES incubations with 9(S)-HPOD (A), ...
Fig. 3. GC-MS analyses of products (Me/TMS) of recombinant NtDES incubations with 9(S)-HPOD (A), 9(S...
Fig. 2. Structural formulae of reaction products of target WT enzymes and their mutant forms. 1, col...
AbstractNon-classical P450s of CYP74 family control several enzymatic conversions of fatty acid hydr...
Fig. 4. The TIC GC–MS chromatograms of products (Me/TMS) of incubations of the recombinant CYP74L2 w...
Fig. 3. The total ion current (TIC) GC–MS chromatograms of products (Me/TMS) of incubations of the r...
AbstractBioinformatics analyses enabled us to identify the hypothetical determinants of catalysis by...
The product specificity and mechanistic peculiarities of two allene oxide synthases, tomato LeAOS3 (...
Fig. 4. GC-MS analyses of products (Me/TMS) of 9(S)-HPOT conversions by recombinant WT ZmAOS1 (А), P...
Fig. 3. Products (Me/TMS) of 9(S)-HPOD (A and B) and 13(S)-HPOD (C and D) conversions by recombinant...
Fig. 2. Structural formulae of reaction products (Me/ TMS) of target WT enzymes and their mutant for...
Fig. 6. The mechanism of fatty acid hydroperoxide conversion by CYP74 proteins showing the role of t...
Toporkova, Yana Y., Smirnova, Elena O., Mukhtarova, Lucia S., Gorina, Svetlana S., Grechkin, Alexand...
Fig. 1. Multiple alignments of partial sequences of following CYP74s: The multiple alignment of diff...
Fig. 5. GC-MS analyses of products (Me/TMS) of LuDES F291V mutant form incubations with 9(S)-HPOD (A...
Fig. 4. GC-MS analyses of products (Me/TMS) of recombinant WT LuDES incubations with 9(S)-HPOD (A), ...
Fig. 3. GC-MS analyses of products (Me/TMS) of recombinant NtDES incubations with 9(S)-HPOD (A), 9(S...
Fig. 2. Structural formulae of reaction products of target WT enzymes and their mutant forms. 1, col...
AbstractNon-classical P450s of CYP74 family control several enzymatic conversions of fatty acid hydr...
Fig. 4. The TIC GC–MS chromatograms of products (Me/TMS) of incubations of the recombinant CYP74L2 w...
Fig. 3. The total ion current (TIC) GC–MS chromatograms of products (Me/TMS) of incubations of the r...
AbstractBioinformatics analyses enabled us to identify the hypothetical determinants of catalysis by...
The product specificity and mechanistic peculiarities of two allene oxide synthases, tomato LeAOS3 (...