Membrane potential based assay for SLC5A7 using HEK-293 SLC5A7 OE cells

FLIPR® membrane potential dye measures changes of charges across the cell membrane, upon activation of SLC5A7. The assay allows the detection of ion channel and transporter modulation by increasing or decreasing the fluorescent signal as cellular membrane potential changes. When cells are depolarized dye enters the cells, causing an increase in fluorescent signal, conversely, cells hyperpolarization results in dye exit and decreased fluorescence. SLC5A7 is a hemicholinium-3 (HC-3)-sensitive transporter that mediates choline uptake by pre-synaptic terminal with a stoichiometry of 1 Na+: 1 choline. Therefore, being this transport electrogenic, it is eligible to be studied using the Membrane Potential dye