Membrane potential based assay for SLC6A6 using HEK-293 SLC6A6 OE cells

FLIPR® membrane potential dye measures changes of charges across the cell membrane, upon activation of SLC6A6. The assay allows the detection of ion channel and transporter modulation by increasing or decreasing the fluorescent signal as cellular membrane potential changes. When cells are depolarized dye enters the cells, causing an increase in fluorescent signal, conversely, cells hyperpolarization results in dye exit and decreased fluorescence. SLC6A6 mediates Taurine uptake in human cells, it is an electrogenic transporter with the stoichiometry 2 Na+:1 Cl-:1 Taurine. As a high-affinity and low-specificity transporter, it has the capability to transport hypotaurine, β-alanine and g-aminobutyrate (GABA), apart from taurine