Diclofenac induced alterations in the phospholipid levels in different tissues of fresh water fish, Channa punctatus

ABSTRACT The extensive usage and improper disposal of pharmaceuticals have the potential to contaminate the water and sediments. Diclofenac is a widely prescribed non-steroidal anti-inflammatory drug that has been frequently detected in surface waters worldwide. There are evidences of its toxicity in aquatic organisms like phytoplankton, zooplankton and fish. The present study aims to investigate the biochemical alterations induced by Diclofenac in fresh water fish, Channa punctatus. Phospholipids, the major biochemical constituents of the cell membrane were taken as biomarkers. The acute toxicity assays were performed by exposing the fish to three different concentrations 5ppm, 25ppm and 50ppm of diclofenac. The effect was observed in different tissues like gill, liver, brain, muscle and kidney after 24h, 48h, 72h, and 96h of exposure with respect to control. Phospholipids were estimated by Zilversmidth and Davis method. Diclofenac exposure on fish at the set concentrations has caused reduction in the phospholipid level of different tissues when compared to control. The findings of the study assume that diclofenac causes biochemical alterations in fish which leads to the impairment of health status of the fish. Key words: Diclofenac, acute toxicity, phospholipids, Channa punctatus REFERENCES Adams SM, Ryon MGA. 1994; A comparison of health assessment approaches for evaluating the effects of contaminant-related stress on fish populations. J Aquat Ecosyst Health, 3: 15-25. Amutha P, Sangeetha G and Mahalingam S. 2002; Diary effluent induced alterations in the protein, carbohydrate and lipid metabolism of a freshwater teleost fish Oreochromis mossambicus. Poll. Res, 21(1): 51-53. Anil Kumar V, Janaiah C and Venkateshwarlu P. 2010; Effect of thiamethoxam alters serum biochemical parameters in Channa punctatus (Bloch) Asian Journal of Bio Science, 5 ( 1) : 106-110. [4].Anusha A, Arunkumar IC, Jayanthi FE, Selvanayagam M. 1996; Quinolphos induced biochemical anomalies in Cirrhinus mrigala (Ham.). J. Environ. Biol., 17(2): 121-124. Arnold KE, Boxall ABA, Brown AR, Cuthbert RJ, Gaw S, Hutchinson TH , Jobling S, Madden JC, Metcalfe CD, Naidoo V, Shore RF, Smits JE, Taggart MA, Thompson HM. 2013; Assessing the exposure risk and impacts of pharmaceuticals in the environment on individuals and ecosystems. Biol Lett, 9(4): 20130492. Bano Y, Hasan M. 1989; Mercury induced time‐dependent alterations in lipid profiles and lipid peroxidation in different body organs of cat‐fish Heteropneustes fossilis. Journal of Environmental Science and Health, Part B: Pesticides, Food Contaminants, and Agricultural Wastes. J Environ Sci Health B. 24(2):145-66. Bell MV, Henderson RJ, and Sargent JR. 1986; The role of polyunsaturated fatty acids in fish. Comp. Biochem. Physiol, 83B: 711-719. Binukumari S , Priyadarsini V and Vasanthi J. 2016; Impact of Diclofenac Drug on the biochemical composition of the fresh water fish, Cirrhinus mrigala Int. J. Adv. Res. Biol. Sci. 3(5): 153-159. Buser HR, Poiger T, and Müller MD. 1998; Occurrence and Fate of the Pharmaceutical Drug Diclofenac in Surface Waters: Rapid Photodegradation in a Lake. Environ. Sci. Technol., 32(22): 3449- 3456. Chovanec A, Hofer R, Schiemer F. Chapter 18 Fish as bioindicators. In: Markert BA, Breure AM and Zechmeister HG. eds. Trace Metals and other Contaminants in the Environment Volume 6, 2003, Bioindicators & Biomonitors — Principles, Concepts and Applications 639-676 .Ezemonye LIN , Ainerua OM , Tongo I. 2014; Changes in glucose and phospholipids level of Oreochromis niloticus fingerlings exposed to varying concentrations of Propoxur. IOSR Journal of Environmental Science, Toxicology and Food Technology, 8 (1) Ver. V : 15-20. Hameed SVS and Muthukumaravel K, 2006; Impact of cadmium on the biochemical constituents of fresh water fish Oreochromis mossambicus. Indian J. Env. Sci., 10(1): 63-65. Javed M and Usmani N. 2015; Stress response of biomolecules (carbohydrate, protein and lipid profiles) in fish Channa punctatus inhabiting river polluted by Thermal Power Plant effluent. Saudi J Biol Sci., 22(2): 237–242. ].Jha BS.1991; Alteration in the protein and lipid content of intestine, liver and gonads in the lead exposed freshwater fish Channa punctatus (Bloch). J. Environ. Ecoplan., 2(3), 281-284. Jones JRE, 1972; In River pollution 2 : Causes and effecst (ed) KL ButterworthJ. Academic Press, New York , p 254 Techniques in Aquatic Toxicology GK Ostrander 2005 p 72 CRC Press, United States of America. Keriko JM, Chege CW , Magu MM , Mwachiro EC , Murigi AN , Githua,M N. and Kareru, P G. 2010; Fish lipid contents and classes of selected fish species found in Lake Naivasha (Kenya) and the fish feeding habits of the lake’s inhabitants African Journal of Pharmacy and Pharmacology, 4(10), 745-753. .Larsson DGJ, de Pedro C, Paxeus N. 2007; Effluent from drug manufactures contains extremely high levels of pharmaceuticals. J Hazard Mater., 148: 751–755. Mukhopadhyay T, Nandi S, and Ghosh S. 2004; Lipid profile and fatty acid composition in eggs of Indian feather back fish phouli (Notopterus pallas) in comparison with body-tissue lipid. Journal of Oleo Science, 53: 323-328. Muley DV, Karanjkar DM and Maske SV. 2007; Impact of industrial effluents on the biochemical composition of fresh water fish Labeo rohita, J. Environ Biol., 28(2):245-249. .Murthy KV, Bhaskar M and Govindappa S. 1994; Studies on lipid profile of fish liver on acclimation to acidic medium. J. Environ. Biol., 15(4), 269-273. Muthukumaravel K , Sivakumar B , Kumarasamy P, and Govindarajan M. 2013; Studies on the toxicity of pesticide monocrotophos on the biochemical constituents of the freshwater fish Labeo rohita. International Journal of Current Biochemistry and Biotechnology, 2 (10): 20-26. .Peake BM, and Braund R. 2009; Environmental Aspects of the Disposal of Pharmaceuticals in New Zealand. Chemistry in New Zealand, 73(2): 58-63. Rao KSP and Rao KVR. 1984; Changes in the tissue lipid profiles of fish (Oreochromis mossambicus) during methyl parathion toxicity--a time course study. Toxicol Lett., 21(2):147-153. Rao KS, Rao KR, Sahib IK, Rao KV. 1985; Combined action of carbaryl and phenthoate on tissue lipid derivatives of murrel, Channa punctatus (Bloch). Ecotoxicol Environ Saf. ;9(1):107-111. Rao KS, and Rao R. 1979; Effect of sublethal concentration of methyl parathion on selected oxidative enzymes and organic matter in tissues of Tilapia mossambica. Curr. Sci., 48(12): 526-528. Rodríguez I, Quintana JB, Carpinteiro J, Carro AM, Lorenzo RA, Cela R. 2003; Determination of acidic drugs in sewage water by gas chromatography-mass 16 spectrometry as tert.-butyldimethylsilyl derivatives. J Chromatogr A, 985: 265-274. Saxena PK , Mani K , Kondal JK. 1986; Effect of safe application rate (SAR) concentrations of some biocides on the gonads of the fresh water murrel, Channa punctatus (Bl.)--a biochemical study. Ecotoxicology and Environmental Safety , 12(1):1-14. Schwaiger J, Ferling H, Mallow U, Wintermayr H, and Negele D.R. 2004; Toxic effects of the non-steroidal anti-inflammatory drug diclofenac Part I: Histopathological alterations and bioaccumulation in rainbow trout. Aquat Toxicol., 68: 141–150. Shoba, K, Poornima A, Harini P and Veeraiah K. 2007; A study on biochemical changes in the fresh water fish, Catla catla (Hamilton) exposed to heavy malathion on the oxidative metabolism of the fish metal toxicant Cadmium chloride. J. Science Engineering and Technology., 1: 4-5. Singh PB. 1992; Impact of malathion and γ-BHC on lipid metabolism in the freshwater female catfish, Heteropneustes fossilis Ecotoxicology and Environmental Safety, 23 (1): 22–32. Singh PB and Singh V. 2006; Impact of endosulfan on the profiles of phospholipids at sublethal concentration in the male Heteropneustes fossilis ( Bloch) Journal of Environmental Biology, 27(3) :509-514. Sivachandran R and Sultana M. 2014; Effect of tannery effluent on lipid profiles in reproductive tissues of freshwater fish Channa straitus . International Journal of Novel Trends in Pharmaceutical Sciences, 4 (4): 77-80. Skoutakis VA, Carter CA, Mickle TR, Smith VH, Arkin CR, Alissandratos J, Petty DE. 1988; Review of diclofenac and evaluation of its place in therapy as a nonsteroidal antiinflammatory agent. Drug Intell Clin Pharm., 22(11): 850-859. Sudhasaravanan R and Binukumari S. 2015; Studies on the Effect of Electroplating Effluent on Biochemical Components of Fish Cyprinus carpio. World Journal of Fish and Marine Sciences 7 (6): 472-478. Vutukuru SS. 2005; Acute Effects of Hexavalent Chromium on Survival, Oxygen Consumption, Hematological Parameters and Some Biochemical Profiles of the Indian Major Carp, Labeo rohita Int. J. Environ. Res. Public Health , 2(3): 456-462. Stalin, Israel S; Das, Sam Manohar S. 2012; Biochemical changes in certain tissues of Cirrhina mrigala (Hamilton) (Cyprinidae:Cyprniformes) exposed to fenthion. International Journal of Environmental Sciences,2(3) : 1268-1277. ].Zilversmidth DB and Davis BS. 1950; Microdetermination of plasma phospholipids by trichloroacetic acid precipitation. J. Lab. Clin. Med, 35: 155-166