Fig. 4 in Flavonoid biosynthesis in Dianthus caryophyllus L. is early regulated during interaction with Fusarium oxysporum f. sp. dianthi

Fig. 4. Important features selected by fold-change (FC) analysis, principal component analysis (PCA) and multivariate empirical bayes approach (MEBA) of carnation samples according to the profiles of constitutive flavonoids from both non-inoculated cultivars. (a) Paired FC analysis with threshold |2| of GCR/MSC ratio as comparison type. UV-based filtered features were labelled retention time–mass/charge ratio (rt/m/z) pairs. A feature was highlighted as significant if this ratio was above/below threshold. Red dots represent features above the threshold and related to GRC; Blue dots represent features below the threshold and related to MSC. (b) PCA-derived 3D-scores plot (81% of explained variance). Cultivars are differentiated by colors (blue = MSC; red = GRC); Sampling times (in h) are differentiated by shapes (circles = 0 h; squares = 12 h; triangles = 24 h; diamonds = 48 h; octagons = 96 h). GRC samples collected at later times (24, 48, and 96 h) are highlighted with the semi-transparent red ellipse. (c) PCA-derived 3D-loadings plot. Each dot represents a feature and the distribution across the 3D-plot is related to its statistical influence on principal components for discriminating the samples. The most-influencing GCR-related features are highlighted the semi-transparent red ellipse. (d) Time-course profiles of those high-ranked features for GRC. GRC = red time-course; MSC = green time-course. (e) Hotelling-T2-ranked features from MEBA. Autoscaled relative abundances per cultivar according to the heat map colors: red = higher; blue = lower. Bold numbers in parenthesis correspond to the annotated features listed in Table 1. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)Published as part of Romero-Rinc, Ana, on, Martínez, Sixta Tulia, Higuera, Blanca Ligia, Coy-Barrera, Ericsson & Ardila, Harold Duban, 2021, Flavonoid biosynthesis in Dianthus caryophyllus L. is early regulated during interaction with Fusarium oxysporum f. sp. dianthi, pp. 1-14 in Phytochemistry (112933) 192 on page 6, DOI: 10.1016/j.phytochem.2021.112933, http://zenodo.org/record/825797