The distribution of putative CSαβ subgroups and putative multi-domain Nemapores reveal additional AMP group diversity across nematode clades.

A: The distribution of four putative CSαβ subgroups [Mollusc/Nematode Defensin (MND), Macin, Traditional Insect Defensin (TID), Drosomycin] in CSαβ-containing nematode species. B: The distribution of putative multi-domain Nemapore-encoding genes across nematode genera. Nematode species/genera are organised by clade [47–48] and superfamily designation (NCBI taxonomy browser; https://www.ncbi.nlm.nih.gov/taxonomy). Black shading indicates the presence of at least one putative AMP gene identified via HMM, BLASTp and tBLASTn analyses. BUSCO complete scores were obtained from WormBase ParaSite V14 [https://parasite.wormbase.org/index.html] [34]. Note that species which do not possess CSαβ encoding genes are not included in A.