Selected regression plots comparing the differential expression values of eight genes in <i>A</i>. <i>hebraeum</i> nymphs, and the unfed male and female tick midguts.

This comparison is between the quantitative real-time PCR (qPCR) data and the RNA-seq data. The log2 fold-change values from qPCR are plotted on the x-axis and the corresponding values from RNA-Seq are plotted on the y-axis. The plot illustrates the correlation and consistency between the qPCR validation and the original RNA-Seq data for these selected genes. All validation plots are detailed in S2 Data.