Additional file 1 of LAST-seq: single-cell RNA sequencing by direct amplification of single-stranded RNA without prior reverse transcription and second-strand synthesis

Additional file 1: Fig. S1. Simulated performance of scRNA-seq with varying single-molecule capture efficiency. Fig. S2. T7 In vitro transcription (IVT) of ssRNA templates. Fig. S3. LAST-seq primer. Fig. S4. Performance of LAST-seq and comparison to SMART-seq. Fig. S5. Comparison between LAST-seq and CEL-seq. Fig. S6. Transcriptional bursting kinetics in human cells. Fig. S7. Chromatin structure and gene expression level in wildtype and SCC4 knockout haploid human cells. Fig. S8. LAST-seq library