Additional file 6: Figure S4. of Application of single-cell RNA sequencing in optimizing a combinatorial therapeutic strategy in metastatic renal cell carcinoma

Performance assessment of single-cell RNA-seq data. Histograms of A singlecell frequencies in the number of generated RNA-seq reads per cell, B uniquely mapped reads, C exonic regional coverage rate, and D detected number of genes per cell. Dashed red lines indicate the mean of the x-axis values. Using filtering criteria of >1Â M reads per cell, >60Â % uniquely mapped rate, >35Â % exonic region coverage rate, and >5000 detected genes, two single cells were not included in subsequent analysis (for details, see Additional file 7: Table S3). (PDF 345 kb