Immunoblot of whole cell lysates from pFliC mutants grown to an OD₆₀₀ of 2.0.

Flagellin is detected by BD Difco Salmonella anti-H a-z antiserum. Ser. Typhimurium 14028s FliCON ΔfliB (chromosomal mutant FliC1) is denoted as “WT.” Wild type ser. Enteritidis P125109 is denoted as “Ent.” Ser. Typhimurium 14028s ΔfliCΔfljBA is denoted as “Fla-.” Numbers 1 through 13 refer to the corresponding pFliC plasmid mutants, as described in Fig 1.