Number of snake skin swab-extracted DNA samples that tested qPCR positive for Ophidiomyces ophidiicola collected in eight different years successfully genotyped and assigned to each clade/clonal lineage using the qPCR-based assay.

Screening of Ophidiomyces ophidiicola in the free-ranging snake community annually harvested for the popular ritual of San Domenico e dei Serpari (Cocullo, AQ, Italy)

Marini, Daniele
Filippi, Ernesto
Montinaro, Gianpaolo
Origgi, Francesco C.

January 2023

In the Abruzzi village of Cocullo (Italy), each year, on May 1st, local snake hunters (known as Serp...

Summary of results of the validated taxon-specific qPCR assays.

Taylor M. Wilcox (394721)
Kellie J. Carim (822254)
Kevin S. McKelvey (394722)
Michael K. Young (394723)
Michael K. Schwartz (108368)

November 2015

Except for the noted problematic polymorphisms, each assay was taxon-specific and amplified all i...

Evaluation of a probe hybridization quantitative polymerase chain reaction assay for Cryptosporidium serpentis in eastern indigo snakes (Drymarchon couperi)

Bogan, J.E.
Wellehan, J.F.X.
Garner, M.M.
Childress, A.L.
Jackson, B.

January 2022

A probe-hybridization quantitative polymerase chain reaction assay specific for Cryptosporidium serp...

Number of snake skin swab-extracted DNA samples that tested qPCR positive for Ophidiomyces ophidiicola collected in eight different years successfully genotyped and assigned to each clade/clonal lineage using the qPCR-based assay.

Ellen Haynes (6832487)
Jeffrey Lorch (16747930)
Matthew C. Allender (7871711)

August 2023

Number of snake skin swab-extracted DNA samples that tested qPCR positive for Ophidiomyces ophidiico...

Number of snake skin swab-extracted DNA samples that tested qPCR positive for Ophidiomyces ophidiicola from 27 snake species successfully genotyped and assigned to each clade/clonal lineage using the qPCR-based assay.

Ellen Haynes (6832487)
Jeffrey Lorch (16747930)
Matthew C. Allender (7871711)

August 2023

Number of snake skin swab-extracted DNA samples that tested qPCR positive for Ophidiomyces ophidiico...

Number of snake skin swab-extracted DNA samples that tested qPCR positive for Ophidiomyces ophidiicola successfully genotyped and assigned to each clade/clonal lineage using the qPCR-based assay from wild snakes from 11 different US states and snakes from managed collections.

Ellen Haynes (6832487)
Jeffrey Lorch (16747930)
Matthew C. Allender (7871711)

August 2023

Number of snake skin swab-extracted DNA samples that tested qPCR positive for Ophidiomyces ophidiico...

Bar graph of number of snake skin swab-extracted DNA samples assigned to each of eight Ophidiomyces ophidiicola clades or clonal lineages.

Ellen Haynes (6832487)
Jeffrey Lorch (16747930)
Matthew C. Allender (7871711)

August 2023

All samples previously tested qPCR positive for O. ophidiicola and were collected from wild snakes (...

Development and application of a qPCR-based genotyping assay for Ophidiomyces ophidiicola to investigate the epidemiology of ophidiomycosis

Haynes, Ellen
Lorch, Jeffrey
Allender, Matthew C.

August 2023

Ophidiomycosis (snake fungal disease) is an infectious disease caused by the fungus Ophidiomyces oph...

DNA concentration by lesion type.

Michelle Waligora Kendall (16747677)
Allison D. Wright (16747680)
Laura A. Adamovicz (7871708)
Kennymac Durante (16747683)
Kirsten E. Andersson (7871705)
Kelcie Frederickson (16747686)
Katie Vivirito (16747689)
Emilie A. Ospina (16747692)
Martha A. Delaney (9720894)
Matthew C. Allender (7871711)

August 2023

DNA concentration (A) and Ophidiomyces ophidiicola qPCR quantity (B) for body swabs collected from p...

Clade or clonal lineage assignment based on qPCR and whole-genome sequencing [17] for 82 DNA extracts of Ophidiomyces ophidiicola and other fungi.

Ellen Haynes (6832487)
Jeffrey Lorch (16747930)
Matthew C. Allender (7871711)

August 2023

Clade or clonal lineage assignment based on qPCR and whole-genome sequencing [17] for 82 DNA extract...

Receiver operating characteristic (ROC) curves for predicting sufficient qPCR amplification for Ophidiomyces ophidiicola clade assignment.

Ellen Haynes (6832487)
Jeffrey Lorch (16747930)
Matthew C. Allender (7871711)

August 2023

Curves are based on (A) DNA concentration (AUC = 0.73), (B) copies of ITS of Ophidiomyces ophidiicol...

Leveraging preserved specimens of Nerodia to infer the spatiotemporal dynamics of Ophidiomyces ophidiicola via quantitative polymerase chain reaction

Stephen F. Harding
MDM Moretta-Urdiales
Stephanie C. Nordmeyer
Elijah Wostl
David Rodriguez

March 2023

Ophidiomyces ophidiicola (Oo) is a fungal pathogen and the causative agent of ophidiomycosis that ha...

Diagnostic characteristics of Ophidiomyces ophidiicola qPCR copy number cutoff values for predicting the presence of skin lesions and the presence of skin fluorescence under ultraviolet light in prairie rattlesnakes (Crotalus viridis) experimentally inoculated with O ophidiicola.

Michelle Waligora Kendall (16747677)
Allison D. Wright (16747680)
Laura A. Adamovicz (7871708)
Kennymac Durante (16747683)
Kirsten E. Andersson (7871705)
Kelcie Frederickson (16747686)
Katie Vivirito (16747689)
Emilie A. Ospina (16747692)
Martha A. Delaney (9720894)
Matthew C. Allender (7871711)

August 2023

Diagnostic characteristics of Ophidiomyces ophidiicola qPCR copy number cutoff values for predicting...

Receiver operating curves.

Michelle Waligora Kendall (16747677)
Allison D. Wright (16747680)
Laura A. Adamovicz (7871708)
Kennymac Durante (16747683)
Kirsten E. Andersson (7871705)
Kelcie Frederickson (16747686)
Katie Vivirito (16747689)
Emilie A. Ospina (16747692)
Martha A. Delaney (9720894)
Matthew C. Allender (7871711)

August 2023

Receiver operating curves for A) Normalized Ophidiomyces ophidiicola qPCR copy number (copies/ng DNA...

Target sequences used to design each of the 11 primer-probe sets used in the qPCR-based genotyping assay for Ophidiomyces ophidiicola and for BLAST-based genotyping of whole genome assemblies of selected samples used in assay validation.

Ellen Haynes (6832487)
Jeffrey Lorch (16747930)
Matthew C. Allender (7871711)

August 2023

Target sequences used to design each of the 11 primer-probe sets used in the qPCR-based genotyping a...

Screening of Ophidiomyces ophidiicola in the free-ranging snake community annually harvested for the popular ritual of San Domenico e dei Serpari (Cocullo, AQ, Italy)

Marini, Daniele
Filippi, Ernesto
Montinaro, Gianpaolo
Origgi, Francesco C.

January 2023

In the Abruzzi village of Cocullo (Italy), each year, on May 1st, local snake hunters (known as Serp...

Summary of results of the validated taxon-specific qPCR assays.

Taylor M. Wilcox (394721)
Kellie J. Carim (822254)
Kevin S. McKelvey (394722)
Michael K. Young (394723)
Michael K. Schwartz (108368)

November 2015

Except for the noted problematic polymorphisms, each assay was taxon-specific and amplified all i...

Evaluation of a probe hybridization quantitative polymerase chain reaction assay for Cryptosporidium serpentis in eastern indigo snakes (Drymarchon couperi)

Bogan, J.E.
Wellehan, J.F.X.
Garner, M.M.
Childress, A.L.
Jackson, B.

January 2022

A probe-hybridization quantitative polymerase chain reaction assay specific for Cryptosporidium serp...

Number of snake skin swab-extracted DNA samples that tested qPCR positive for Ophidiomyces ophidiicola collected in eight different years successfully genotyped and assigned to each clade/clonal lineage using the qPCR-based assay.

Ellen Haynes (6832487)
Jeffrey Lorch (16747930)
Matthew C. Allender (7871711)

August 2023

Number of snake skin swab-extracted DNA samples that tested qPCR positive for Ophidiomyces ophidiico...

Number of snake skin swab-extracted DNA samples that tested qPCR positive for Ophidiomyces ophidiicola from 27 snake species successfully genotyped and assigned to each clade/clonal lineage using the qPCR-based assay.

Ellen Haynes (6832487)
Jeffrey Lorch (16747930)
Matthew C. Allender (7871711)

August 2023

Number of snake skin swab-extracted DNA samples that tested qPCR positive for Ophidiomyces ophidiico...

Number of snake skin swab-extracted DNA samples that tested qPCR positive for Ophidiomyces ophidiicola successfully genotyped and assigned to each clade/clonal lineage using the qPCR-based assay from wild snakes from 11 different US states and snakes from managed collections.

Ellen Haynes (6832487)
Jeffrey Lorch (16747930)
Matthew C. Allender (7871711)

August 2023

Number of snake skin swab-extracted DNA samples that tested qPCR positive for Ophidiomyces ophidiico...

Bar graph of number of snake skin swab-extracted DNA samples assigned to each of eight Ophidiomyces ophidiicola clades or clonal lineages.

Ellen Haynes (6832487)
Jeffrey Lorch (16747930)
Matthew C. Allender (7871711)

August 2023

All samples previously tested qPCR positive for O. ophidiicola and were collected from wild snakes (...

Development and application of a qPCR-based genotyping assay for Ophidiomyces ophidiicola to investigate the epidemiology of ophidiomycosis

Haynes, Ellen
Lorch, Jeffrey
Allender, Matthew C.

August 2023

Ophidiomycosis (snake fungal disease) is an infectious disease caused by the fungus Ophidiomyces oph...

DNA concentration by lesion type.

Michelle Waligora Kendall (16747677)
Allison D. Wright (16747680)
Laura A. Adamovicz (7871708)
Kennymac Durante (16747683)
Kirsten E. Andersson (7871705)
Kelcie Frederickson (16747686)
Katie Vivirito (16747689)
Emilie A. Ospina (16747692)
Martha A. Delaney (9720894)
Matthew C. Allender (7871711)

August 2023

DNA concentration (A) and Ophidiomyces ophidiicola qPCR quantity (B) for body swabs collected from p...

Clade or clonal lineage assignment based on qPCR and whole-genome sequencing [17] for 82 DNA extracts of Ophidiomyces ophidiicola and other fungi.

Ellen Haynes (6832487)
Jeffrey Lorch (16747930)
Matthew C. Allender (7871711)

August 2023

Clade or clonal lineage assignment based on qPCR and whole-genome sequencing [17] for 82 DNA extract...

Receiver operating characteristic (ROC) curves for predicting sufficient qPCR amplification for Ophidiomyces ophidiicola clade assignment.

Ellen Haynes (6832487)
Jeffrey Lorch (16747930)
Matthew C. Allender (7871711)

August 2023

Curves are based on (A) DNA concentration (AUC = 0.73), (B) copies of ITS of Ophidiomyces ophidiicol...

Leveraging preserved specimens of Nerodia to infer the spatiotemporal dynamics of Ophidiomyces ophidiicola via quantitative polymerase chain reaction

Stephen F. Harding
MDM Moretta-Urdiales
Stephanie C. Nordmeyer
Elijah Wostl
David Rodriguez

March 2023

Ophidiomyces ophidiicola (Oo) is a fungal pathogen and the causative agent of ophidiomycosis that ha...

Diagnostic characteristics of Ophidiomyces ophidiicola qPCR copy number cutoff values for predicting the presence of skin lesions and the presence of skin fluorescence under ultraviolet light in prairie rattlesnakes (Crotalus viridis) experimentally inoculated with O ophidiicola.

Michelle Waligora Kendall (16747677)
Allison D. Wright (16747680)
Laura A. Adamovicz (7871708)
Kennymac Durante (16747683)
Kirsten E. Andersson (7871705)
Kelcie Frederickson (16747686)
Katie Vivirito (16747689)
Emilie A. Ospina (16747692)
Martha A. Delaney (9720894)
Matthew C. Allender (7871711)

August 2023

Diagnostic characteristics of Ophidiomyces ophidiicola qPCR copy number cutoff values for predicting...

Receiver operating curves.

Michelle Waligora Kendall (16747677)
Allison D. Wright (16747680)
Laura A. Adamovicz (7871708)
Kennymac Durante (16747683)
Kirsten E. Andersson (7871705)
Kelcie Frederickson (16747686)
Katie Vivirito (16747689)
Emilie A. Ospina (16747692)
Martha A. Delaney (9720894)
Matthew C. Allender (7871711)

August 2023

Receiver operating curves for A) Normalized Ophidiomyces ophidiicola qPCR copy number (copies/ng DNA...

Target sequences used to design each of the 11 primer-probe sets used in the qPCR-based genotyping assay for Ophidiomyces ophidiicola and for BLAST-based genotyping of whole genome assemblies of selected samples used in assay validation.

Ellen Haynes (6832487)
Jeffrey Lorch (16747930)
Matthew C. Allender (7871711)

August 2023

Target sequences used to design each of the 11 primer-probe sets used in the qPCR-based genotyping a...

Screening of Ophidiomyces ophidiicola in the free-ranging snake community annually harvested for the popular ritual of San Domenico e dei Serpari (Cocullo, AQ, Italy)

Marini, Daniele
Filippi, Ernesto
Montinaro, Gianpaolo
Origgi, Francesco C.

January 2023

In the Abruzzi village of Cocullo (Italy), each year, on May 1st, local snake hunters (known as Serp...

Summary of results of the validated taxon-specific qPCR assays.

Taylor M. Wilcox (394721)
Kellie J. Carim (822254)
Kevin S. McKelvey (394722)
Michael K. Young (394723)
Michael K. Schwartz (108368)

November 2015

Except for the noted problematic polymorphisms, each assay was taxon-specific and amplified all i...

Evaluation of a probe hybridization quantitative polymerase chain reaction assay for Cryptosporidium serpentis in eastern indigo snakes (Drymarchon couperi)

Bogan, J.E.
Wellehan, J.F.X.
Garner, M.M.
Childress, A.L.
Jackson, B.

January 2022

A probe-hybridization quantitative polymerase chain reaction assay specific for Cryptosporidium serp...

Number of snake skin swab-extracted DNA samples that tested qPCR positive for <i>Ophidiomyces ophidiicola</i> collected in eight different years successfully genotyped and assigned to each clade/clonal lineage using the qPCR-based assay.

Abstract

Extracted data

Number of snake skin swab-extracted DNA samples that tested qPCR positive for <i>Ophidiomyces ophidiicola</i> collected in eight different years successfully genotyped and assigned to each clade/clonal lineage using the qPCR-based assay.

Abstract

Extracted data

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