Amino acid conservation of the folded domains and IDRs of Cno and Afadin.

For each folded domain and for the IDR and FAB we calculated amino acid identity. Comparisons were made relative to Drosophila melanogaster Cno (relative to other insects—all except Tribolium and Heliconius are in the Order Diptera) or human Afadin (relative to other vertebrates and one non-vertebrate chordate, Amphioxus). Color coding indicates ranges of sequence identity. Green = 85–100% identical. Blue = 70–84% identical. Yellow = 55–69% identical. Red = <55% identical. Small insertion/deletion polymorphisms (indels) are noted. “No significant similarity” means the program Blast found no match.