mAb escape heatmaps for (A) Ly-CoV016, (B) REGN 10987 and (C) REGN 10933.

To the immediate right of each heatmap are plots showing pseudovirus neutralisation curves for a selected panel of pseudovirus mutants. To the furthest right are structural representations of the respective mAb binding to the RBD, with predicted positions of escape highlighted. The RBD positions presented in the heatmap are those where escape occurs most frequently in the mAbs. WT = wild type amino acid. Blank squares represent amino acids not present in the library. Adjusted enrichment is the enrichment score multiplied by the fraction of amino acids other than WT that would be predicted to escape. (D) Pseudovirus neutralisation curves for point mutations S477D and S477P on an Alpha or Wuhan(D614G) pseudovirus compared to WT Alpha and Wuhan(D614G) pseudovirus. All neutralisation assays were performed in duplicate. (E) Structural representation of REGN 10933 binding to SARS-CoV-2 RBD. PDB: 6XDG [55]. HIV based pseudovirus platform with hACE2 over-expressing HEK-293T cells used for neutralisation assays.