Analysis of total Ub in subcellular fractions and pS65-Ub upon oxidative stress-induction.

(A) Representative immunoblot of the subcellular fractionation of 2-day-old flies with the transgenic expression of ntc or USP30 RNAi with the ubiquitous driver da-GAL4. Cytosolic- and mitochondria-enriched fractions are label with Actin and ATP5a, respectively. (B) Representative immunoblot of total ubiquitin (FK2) of 2-day-old flies in control, ntc overexpression and USP30 knockdown with the ubiquitous driver da-GAL4. (C) Representative immunoblot of pS65-Ub levels of 2-day-old flies treated with paraquat (PQ) in control and ntc mutants. (TIFF)